2v4a

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[[Image:2v4a.gif|left|200px]]
[[Image:2v4a.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2v4a |SIZE=350|CAPTION= <scene name='initialview01'>2v4a</scene>, resolution 1.93&Aring;
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The line below this paragraph, containing "STRUCTURE_2v4a", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:Cl+Binding+Site+For+Chain+C'>AC3</scene>, <scene name='pdbsite=AC4:Cl+Binding+Site+For+Chain+D'>AC4</scene>, <scene name='pdbsite=AC5:Gol+Binding+Site+For+Chain+C'>AC5</scene>, <scene name='pdbsite=AC6:Gol+Binding+Site+For+Chain+B'>AC6</scene>, <scene name='pdbsite=AC7:Gol+Binding+Site+For+Chain+A'>AC7</scene>, <scene name='pdbsite=AC8:Gol+Binding+Site+For+Chain+D'>AC8</scene>, <scene name='pdbsite=AC9:Dms+Binding+Site+For+Chain+B'>AC9</scene>, <scene name='pdbsite=BC1:Dms+Binding+Site+For+Chain+B'>BC1</scene> and <scene name='pdbsite=BC2:So4+Binding+Site+For+Chain+A'>BC2</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2v4a| PDB=2v4a | SCENE= }}
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|RELATEDENTRY=[[2jig|2JIG]], [[2jij|2JIJ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v4a OCA], [http://www.ebi.ac.uk/pdbsum/2v4a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v4a RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF THE SEMET-LABELED PROLYL-4 HYDROXYLASE (P4H) TYPE I FROM GREEN ALGAE CHLAMYDOMONAS REINHARDTII.'''
'''CRYSTAL STRUCTURE OF THE SEMET-LABELED PROLYL-4 HYDROXYLASE (P4H) TYPE I FROM GREEN ALGAE CHLAMYDOMONAS REINHARDTII.'''
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[[Category: Myllyharju, J.]]
[[Category: Myllyharju, J.]]
[[Category: Wierenga, R K.]]
[[Category: Wierenga, R K.]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:12:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:08:21 2008''
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Revision as of 15:12, 4 May 2008

Image:2v4a.gif

Template:STRUCTURE 2v4a

CRYSTAL STRUCTURE OF THE SEMET-LABELED PROLYL-4 HYDROXYLASE (P4H) TYPE I FROM GREEN ALGAE CHLAMYDOMONAS REINHARDTII.


Overview

Prolyl 4-hydroxylases (P4Hs) are 2-oxoglutarate dioxygenases that catalyze the hydroxylation of peptidyl prolines. They play an important role in collagen synthesis, oxygen homeostasis, and plant cell wall formation. We describe four structures of a P4H from the green alga Chlamydomonas reinhardtii, two of the apoenzyme at 1.93 and 2.90 A resolution, one complexed with the competitive inhibitor Zn2+, and one with Zn2+ and pyridine 2,4-dicarboxylate (which is an analogue of 2-oxoglutarate) at 1.85 A resolution. The structures reveal the double-stranded beta-helix core fold (jellyroll motif), typical for 2-oxoglutarate dioxygenases. The catalytic site is at the center of an extended shallow groove lined by two flexible loops. Mutagenesis studies together with the crystallographic data indicate that this groove participates in the binding of the proline-rich peptide-substrates. It is discussed that the algal P4H and the catalytic domain of collagen P4Hs have notable structural similarities, suggesting that these enzymes form a separate structural subgroup of P4Hs different from the hypoxia-inducible factor P4Hs. Key structural differences between these two subgroups are described. These studies provide first insight into the structure-function relationships of the collagen P4Hs, which unlike the hypoxia-inducible factor P4Hs use proline-rich peptides as their substrates.

About this Structure

2V4A is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

Reference

The active site of an algal prolyl 4-hydroxylase has a large structural plasticity., Koski MK, Hieta R, Bollner C, Kivirikko KI, Myllyharju J, Wierenga RK, J Biol Chem. 2007 Dec 21;282(51):37112-23. Epub 2007 Oct 16. PMID:17940281 Page seeded by OCA on Sun May 4 18:12:04 2008

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