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2v9v
From Proteopedia
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'''CRYSTAL STRUCTURE OF MOORELLA THERMOACETICA SELB(377-511)''' | '''CRYSTAL STRUCTURE OF MOORELLA THERMOACETICA SELB(377-511)''' | ||
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[[Category: Ganichkin, O.]] | [[Category: Ganichkin, O.]] | ||
[[Category: Wahl, M C.]] | [[Category: Wahl, M C.]] | ||
| - | [[Category: | + | [[Category: Cytoplasm]] |
| - | [[Category: | + | [[Category: Gtp-binding]] |
| - | [[Category: | + | [[Category: Nucleotide-binding]] |
| - | [[Category: | + | [[Category: Protein biosynthesis]] |
| - | [[Category: | + | [[Category: Protein conformational change]] |
| - | [[Category: | + | [[Category: Protein dynamic]] |
| - | [[Category: | + | [[Category: Selenocysteine]] |
| - | [[Category: | + | [[Category: Selenoprotein biosynthesis]] |
| - | [[Category: | + | [[Category: Transcription elongation factor selb]] |
| - | [[Category: | + | [[Category: Winged-helix domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:27:34 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:27, 4 May 2008
CRYSTAL STRUCTURE OF MOORELLA THERMOACETICA SELB(377-511)
Overview
The crystal structure of the first two winged-helix motifs of translation elongation factor SelB from Moorella thermoacetica has been determined at 1.1 A resolution. Compared with the previous structure of the two domains in conjunction with winged-helix modules 3 and 4, the first winged-helix domain underwent a substantial conformational change during which the alpha-helical and beta-sheet portions of the element opened up like a shell. This conformational rearrangement was elicited by a change in the orientation of Trp396, leading to the disclosure of a bona fide ligand-binding site in the direct vicinity of Trp396. Additionally, the C-terminal tail of the second domain followed a different path compared with the previous structure. It is conceivable that these conformational switches constitute part of the molecular mechanism that underlies the communication between the N-terminal part of SelB, which binds Sec-tRNA(Sec) and GTP, and the C-terminal part of the protein, which binds selenocysteine-insertion sequences.
About this Structure
2V9V is a Single protein structure of sequence from Moorella thermoacetica. Full crystallographic information is available from OCA.
Reference
Conformational switches in winged-helix domains 1 and 2 of bacterial translation elongation factor SelB., Ganichkin O, Wahl MC, Acta Crystallogr D Biol Crystallogr. 2007 Oct;63(Pt 10):1075-81. Epub 2007, Sep 19. PMID:17881825 Page seeded by OCA on Sun May 4 18:27:34 2008
Categories: Moorella thermoacetica | Single protein | Ganichkin, O. | Wahl, M C. | Cytoplasm | Gtp-binding | Nucleotide-binding | Protein biosynthesis | Protein conformational change | Protein dynamic | Selenocysteine | Selenoprotein biosynthesis | Transcription elongation factor selb | Winged-helix domain
