6i1r
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of CMP bound CST in an outward facing conformation== | |
| + | <StructureSection load='6i1r' size='340' side='right'caption='[[6i1r]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6i1r]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I1R FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i1r OCA], [http://pdbe.org/6i1r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i1r RCSB], [http://www.ebi.ac.uk/pdbsum/6i1r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i1r ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The decoration of secretory glycoproteins and glycolipids with sialic acid is critical to many physiological and pathological processes. Sialyation is dependent on a continuous supply of sialic acid into Golgi organelles in the form of CMP-sialic acid. Translocation of CMP-sialic acid into Golgi is carried out by the CMP-sialic acid transporter (CST). Mutations in human CST are linked to glycosylation disorders, and CST is important for glycopathway engineering, as it is critical for sialyation efficiency of therapeutic glycoproteins. The mechanism of how CMP-sialic acid is recognized and translocated across Golgi membranes in exchange for CMP is poorly understood. Here we have determined the crystal structure of a Zea mays CST in complex with CMP. We conclude that the specificity of CST for CMP-sialic acid is established by the recognition of the nucleotide CMP to such an extent that they are mechanistically capable of both passive and coupled antiporter activity. | ||
| - | + | Structural basis for the delivery of activated sialic acid into Golgi for sialyation.,Nji E, Gulati A, Qureshi AA, Coincon M, Drew D Nat Struct Mol Biol. 2019 May 27. pii: 10.1038/s41594-019-0225-y. doi:, 10.1038/s41594-019-0225-y. PMID:31133698<ref>PMID:31133698</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6i1r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Drew, D]] | ||
| + | [[Category: Gulati, A]] | ||
| + | [[Category: Nji, E]] | ||
| + | [[Category: Qureshi, A A]] | ||
| + | [[Category: Cmp-sialic acid transporter]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Nucleotide sugar transporter]] | ||
| + | [[Category: Secondary active transporter]] | ||
| + | [[Category: Slc35a1]] | ||
Revision as of 22:46, 5 June 2019
Crystal structure of CMP bound CST in an outward facing conformation
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