2vka

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[[Image:2vka.jpg|left|200px]]
[[Image:2vka.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2vka |SIZE=350|CAPTION= <scene name='initialview01'>2vka</scene>, resolution 2.000&Aring;
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The line below this paragraph, containing "STRUCTURE_2vka", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Hem+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Ca+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Ca+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:Gol+Binding+Site+For+Chain+A'>AC5</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2vka| PDB=2vka | SCENE= }}
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|RELATEDENTRY=[[2boq|2BOQ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vka OCA], [http://www.ebi.ac.uk/pdbsum/2vka PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2vka RCSB]</span>
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}}
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'''SITE-DIRECTED MUTAGENESIS OF THE CATALYTIC TRYPTOPHAN ENVIRONMENT IN PLEUROTUS ERYNGII VERSATILE PEROXIDASE'''
'''SITE-DIRECTED MUTAGENESIS OF THE CATALYTIC TRYPTOPHAN ENVIRONMENT IN PLEUROTUS ERYNGII VERSATILE PEROXIDASE'''
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[[Category: Ruiz-Duenas, F J.]]
[[Category: Ruiz-Duenas, F J.]]
[[Category: Smith, A.]]
[[Category: Smith, A.]]
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[[Category: allelic variant]]
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[[Category: Allelic variant]]
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[[Category: aromatic-substrate binding]]
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[[Category: Aromatic-substrate binding]]
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[[Category: class ii ( fungal) peroxidase]]
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[[Category: Electron transfer]]
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[[Category: electron transfer]]
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[[Category: Homology modeling]]
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[[Category: homology modeling]]
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[[Category: Lignin degradation]]
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[[Category: lignin degradation]]
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[[Category: Lignin peroxidase]]
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[[Category: lignin peroxidase]]
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[[Category: Manganese peroxidase]]
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[[Category: manganese peroxidase]]
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[[Category: Mn-independent oxidation phenolic non-phenolic aromatic]]
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[[Category: mn-independent oxidation phenolic non-phenolic aromatic]]
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[[Category: Mnii oxidation]]
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[[Category: mnii oxidation]]
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[[Category: Oxidoreductase]]
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[[Category: oxidoreductase]]
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[[Category: Peroxidase]]
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[[Category: peroxidase]]
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[[Category: Polyvalent peroxidase]]
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[[Category: polyvalent peroxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:57:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:13:16 2008''
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Revision as of 15:57, 4 May 2008

Image:2vka.jpg

Template:STRUCTURE 2vka

SITE-DIRECTED MUTAGENESIS OF THE CATALYTIC TRYPTOPHAN ENVIRONMENT IN PLEUROTUS ERYNGII VERSATILE PEROXIDASE


Overview

Lignin degradation by fungal peroxidases is initiated by one-electron transfer to an exposed tryptophan radical, a reaction mediated by veratryl alcohol (VA) in lignin peroxidase (LiP). Versatile peroxidase (VP) differs not only in its oxidation of Mn2+ at a second catalytic site but also in its ability to directly oxidize different aromatic compounds. The catalytic tryptophan environment was compared in LiP and VP crystal structures, and six residues near VP Trp164 were modified by site-directed mutagenesis. Oxidation of Mn2+ was practically unaffected. However, several mutations modified the oxidation kinetics of the high-redox-potential substrates VA and Reactive Black 5 (RB5), demonstrating that other residues contribute to substrate oxidation by the Trp164 radical. Introducing acidic residues at the tryptophan environment did not increase the efficiency of VP oxidizing VA. On the contrary, all variants harboring the R257D mutation lost their activity on RB5. Interestingly, this activity was restored when VA was added as a mediator, revealing the LiP-type behavior of this variant. Moreover, combination of the A260F and R257A mutations strongly increased (20-50-fold) the apparent second-order rate constants for reduction of VP compounds I and II by VA to values similar to those found in LiP. Dissociation of the enzyme-product complex seemed to be the limiting step in the turnover of this improved variant. Nonexposed residues in the vicinity of Trp164 can also affect VP activity, as found with the M247F mutation. This was a direct effect since no modification of the surrounding residues was found in the crystal structure of this variant.

About this Structure

2VKA is a Single protein structure of sequence from Pleurotus eryngii. Full crystallographic information is available from OCA.

Reference

Site-Directed Mutagenesis of the Catalytic Tryptophan Environment in Pleurotus eryngii Versatile Peroxidase(,)., Ruiz-Duenas FJ, Morales M, Mate MJ, Romero A, Martinez MJ, Smith AT, Martinez AT, Biochemistry. 2008 Feb 12;47(6):1685-95. Epub 2008 Jan 18. PMID:18201105 Page seeded by OCA on Sun May 4 18:57:17 2008

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