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| | ==Crystal structure of E. coli DExH-box NTPase HrpB== | | ==Crystal structure of E. coli DExH-box NTPase HrpB== |
| - | <StructureSection load='6eud' size='340' side='right' caption='[[6eud]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='6eud' size='340' side='right'caption='[[6eud]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6eud]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EUD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EUD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6eud]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EUD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EUD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hrpB, yadO, b0148, JW0144 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eud FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eud OCA], [https://pdbe.org/6eud PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eud RCSB], [https://www.ebi.ac.uk/pdbsum/6eud PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eud ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eud FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eud OCA], [http://pdbe.org/6eud PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eud RCSB], [http://www.ebi.ac.uk/pdbsum/6eud PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eud ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/HRPB_ECOLI HRPB_ECOLI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6eud" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6eud" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Helicase 3D structures|Helicase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| - | [[Category: RNA helicase]] | + | [[Category: Large Structures]] |
| - | [[Category: Pietrzyk-Brzezinska, A J]] | + | [[Category: Pietrzyk-Brzezinska AJ]] |
| - | [[Category: Wahl, M C]] | + | [[Category: Wahl MC]] |
| - | [[Category: Bacterial helicase]]
| + | |
| - | [[Category: Deah/rha helicase]]
| + | |
| - | [[Category: Hrpb]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| Structural highlights
Function
HRPB_ECOLI
Publication Abstract from PubMed
Eukaryotic DExH-box proteins are important post-transcriptional gene regulators, many of which employ RNA-stimulated nucleoside triphosphatase activity to remodel RNAs or ribonucleoprotein complexes. However, bacterial DExH-box proteins are structurally and functionally poorly characterized. We report the crystal structure of the Escherichia coli DExH-box protein HrpB. A globular head is composed of dual RecA, winged-helix, helical bundle and oligonucleotide/oligosaccharide-binding domains, resembling a compact version of eukaryotic DExH-box proteins. Additionally, HrpB harbors a C-terminal region not found in proteins with known structure, which bestows the protein with unique interaction potential. Interaction and activity assays showed that the protein binds RNA but not DNA, hydrolyzes all nucleoside triphosphates in an RNA-stimulated manner, but does not unwind diverse model RNAs in vitro. These observations can be rationalized by detailed comparisons with structurally characterized eukaryotic DExH-box proteins. Comparative phenotypic analyses of an E. coli hrpB knockout mutant suggested diverse functions of HrpB homologs in different bacteria.
Crystal Structure of the Escherichia coli DExH-Box NTPase HrpB.,Pietrzyk-Brzezinska AJ, Absmeier E, Klauck E, Wen Y, Antelmann H, Wahl MC Structure. 2018 Aug 13. pii: S0969-2126(18)30259-4. doi:, 10.1016/j.str.2018.07.013. PMID:30174149[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pietrzyk-Brzezinska AJ, Absmeier E, Klauck E, Wen Y, Antelmann H, Wahl MC. Crystal Structure of the Escherichia coli DExH-Box NTPase HrpB. Structure. 2018 Aug 13. pii: S0969-2126(18)30259-4. doi:, 10.1016/j.str.2018.07.013. PMID:30174149 doi:http://dx.doi.org/10.1016/j.str.2018.07.013
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