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5ydw
From Proteopedia
(Difference between revisions)
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==Full-length structure of HypT from Salmonella typhimuriuma (hypochlorite-specific LysR-type transcriptional regulator)== | ==Full-length structure of HypT from Salmonella typhimuriuma (hypochlorite-specific LysR-type transcriptional regulator)== | ||
| - | <StructureSection load='5ydw' size='340' side='right' caption='[[5ydw]], [[Resolution|resolution]] 3.30Å' scene=''> | + | <StructureSection load='5ydw' size='340' side='right'caption='[[5ydw]], [[Resolution|resolution]] 3.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5ydw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YDW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YDW FirstGlance]. <br> | <table><tr><td colspan='2'>[[5ydw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YDW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YDW FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ydw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ydw OCA], [http://pdbe.org/5ydw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ydw RCSB], [http://www.ebi.ac.uk/pdbsum/5ydw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ydw ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ydw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ydw OCA], [http://pdbe.org/5ydw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ydw RCSB], [http://www.ebi.ac.uk/pdbsum/5ydw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ydw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hypochlorous acid (HOCl) is generated in the immune system to kill microorganisms. In Escherichia coli, a hypochlorite-specific transcription regulator, HypT, has been characterized. HypT belongs to the LysR-type transcriptional regulator (LTTR) family that contains a DNA-binding domain (DBD) and a regulatory domain (RD). Here, we identified a hypT gene from Salmonella enterica serovar Typhimurium and determined crystal structures of the full-length HypT protein and the RD. The full-length structure reveals a type of tetrameric assembly in the LTTR family. Based on HOCl-bound and oxidation-mimicking structures, we identified a HOCl-driven methionine oxidation mechanism, in which the bound HOCl oxidizes a conserved methionine residue lining the putative ligand-binding site in the RD. Furthermore, we proposed a molecular model for the oxidized HypT, where methionine oxidation by HOCl results in a conformational change of the RD, inducing a counter rotation of the DBD dimers. Target genes that are regulated by HypT and their roles in Salmonella were also investigated. DNase I footprinting experiments revealed a DNA segment containing two pseudopalindromic motifs that are separated by approximately 100 bp, suggesting that only the oxidized structure makes a concomitant binding, forming a DNA loop. An understanding of the HypT-mediated mechanism would be helpful for controlling many pathogenic bacteria by counteracting bacterial HOCl defense mechanisms. | ||
| + | |||
| + | Structural basis for HOCl recognition and regulation mechanisms of HypT, a hypochlorite-specific transcriptional regulator.,Jo I, Kim D, No T, Hong S, Ahn J, Ryu S, Ha NC Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3740-3745. doi:, 10.1073/pnas.1811509116. Epub 2019 Feb 7. PMID:30733296<ref>PMID:30733296</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5ydw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bacillus typhimurium loeffler 1892]] | [[Category: Bacillus typhimurium loeffler 1892]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Ahn, J]] | [[Category: Ahn, J]] | ||
[[Category: Ha, N C]] | [[Category: Ha, N C]] | ||
Revision as of 06:32, 17 April 2019
Full-length structure of HypT from Salmonella typhimuriuma (hypochlorite-specific LysR-type transcriptional regulator)
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