2z48

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[[Image:2z48.gif|left|200px]]
[[Image:2z48.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2z48 |SIZE=350|CAPTION= <scene name='initialview01'>2z48</scene>, resolution 1.70&Aring;
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The line below this paragraph, containing "STRUCTURE_2z48", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:A2g+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Nga+Binding+Site+For+Residue+A+1002'>AC2</scene>, <scene name='pdbsite=AC3:Nga+Binding+Site+For+Residue+A+1003'>AC3</scene>, <scene name='pdbsite=AC4:Nga+Binding+Site+For+Residue+A+1004'>AC4</scene>, <scene name='pdbsite=AC5:A2g+Binding+Site+For+Residue+A+1005'>AC5</scene>, <scene name='pdbsite=AC6:Nga+Binding+Site+For+Residue+A+1006'>AC6</scene>, <scene name='pdbsite=AC7:A2g+Binding+Site+For+Residue+B+2001'>AC7</scene>, <scene name='pdbsite=AC8:Nga+Binding+Site+For+Residue+B+2002'>AC8</scene>, <scene name='pdbsite=AC9:Nga+Binding+Site+For+Residue+B+2003'>AC9</scene>, <scene name='pdbsite=BC1:Nga+Binding+Site+For+Residue+B+2004'>BC1</scene>, <scene name='pdbsite=BC2:A2g+Binding+Site+For+Residue+B+2005'>BC2</scene>, <scene name='pdbsite=BC3:Nga+Binding+Site+For+Residue+B+2006'>BC3</scene>, <scene name='pdbsite=BC4:Ca+Binding+Site+For+Residue+A+1007'>BC4</scene>, <scene name='pdbsite=BC5:Ca+Binding+Site+For+Residue+A+1102'>BC5</scene>, <scene name='pdbsite=BC6:Ca+Binding+Site+For+Residue+A+1103'>BC6</scene>, <scene name='pdbsite=BC7:Ca+Binding+Site+For+Residue+A+1104'>BC7</scene>, <scene name='pdbsite=BC8:Ca+Binding+Site+For+Residue+A+1205'>BC8</scene>, <scene name='pdbsite=BC9:Ca+Binding+Site+For+Residue+B+1201'>BC9</scene>, <scene name='pdbsite=CC1:Ca+Binding+Site+For+Residue+B+1202'>CC1</scene>, <scene name='pdbsite=CC2:Ca+Binding+Site+For+Residue+B+1203'>CC2</scene>, <scene name='pdbsite=CC3:Ca+Binding+Site+For+Residue+B+1204'>CC3</scene>, <scene name='pdbsite=CC4:Ca+Binding+Site+For+Residue+B+1205'>CC4</scene>, <scene name='pdbsite=CC5:Mg+Binding+Site+For+Residue+A+1111'>CC5</scene>, <scene name='pdbsite=CC6:Mg+Binding+Site+For+Residue+A+1112'>CC6</scene>, <scene name='pdbsite=CC7:Mg+Binding+Site+For+Residue+B+1211'>CC7</scene> and <scene name='pdbsite=CC8:Mg+Binding+Site+For+Residue+B+1212'>CC8</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2z48| PDB=2z48 | SCENE= }}
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|RELATEDENTRY=[[1vcl|1VCL]], [[2z49|2Z49]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z48 OCA], [http://www.ebi.ac.uk/pdbsum/2z48 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z48 RCSB]</span>
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}}
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'''Crystal Structure of Hemolytic Lectin CEL-III Complexed with GalNac'''
'''Crystal Structure of Hemolytic Lectin CEL-III Complexed with GalNac'''
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[[Category: Uchida, T.]]
[[Category: Uchida, T.]]
[[Category: Unno, H.]]
[[Category: Unno, H.]]
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[[Category: calcium]]
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[[Category: Calcium]]
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[[Category: cel-iii]]
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[[Category: Cel-iii]]
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[[Category: galnac]]
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[[Category: Galnac]]
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[[Category: hemagglutination]]
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[[Category: Hemagglutination]]
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[[Category: hemolysis]]
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[[Category: Hemolysis]]
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[[Category: lectin]]
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[[Category: Lectin]]
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[[Category: magnesium]]
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[[Category: Magnesium]]
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[[Category: pore-forming]]
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[[Category: Pore-forming]]
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[[Category: toxin]]
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[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 19:56:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:18:21 2008''
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Revision as of 16:56, 4 May 2008

Image:2z48.gif

Template:STRUCTURE 2z48

Crystal Structure of Hemolytic Lectin CEL-III Complexed with GalNac


Overview

CEL-III is a Ca(2+)-dependent hemolytic lectin, isolated from the marine invertebrate Cucumaria echinata. The three-dimensional structure of CEL-III/GalNAc and CEL-III/methyl alpha-galactoside complexes was solved by x-ray crystallographic analysis. In these complexes, five carbohydrate molecules were found to be bound to two carbohydrate-binding domains (domains 1 and 2) located in the N-terminal 2/3 portion of the polypeptide and that contained beta-trefoil folds similar to ricin B-chain. The 3-OH and 4-OH of bound carbohydrate molecules were coordinated with Ca(2+) located at the subdomains 1alpha, 1gamma, 2alpha, 2beta, and 2gamma, simultaneously forming hydrogen bond networks with nearby amino acid side chains, which is similar to carbohydrate binding in C-type lectins. The binding of carbohydrates was further stabilized by aromatic amino acid residues, such as tyrosine and tryptophan, through a stacking interaction with the hydrophobic face of carbohydrates. The importance of amino acid residues in the carbohydrate-binding sites was confirmed by the mutational analyses. The orientation of bound GalNAc and methyl alpha-galactoside was similar to the galactose moiety of lactose bound to the carbohydrate-binding site of the ricin B-chain, although the ricin B-chain does not require Ca(2+) ions for carbohydrate binding. The binding of the carbohydrates induced local structural changes in carbohydrate-binding sites in subdomains 2alpha and 2beta. Binding of GalNAc also induced a slight change in the main chain structure of domain 3, which could be related to the conformational change upon binding of specific carbohydrates to induce oligomerization of the protein.

About this Structure

2Z48 is a Single protein structure of sequence from Cucumaria echinata. Full crystallographic information is available from OCA.

Reference

C-type lectin-like carbohydrate recognition of the hemolytic lectin CEL-III containing ricin-type -trefoil folds., Hatakeyama T, Unno H, Kouzuma Y, Uchida T, Eto S, Hidemura H, Kato N, Yonekura M, Kusunoki M, J Biol Chem. 2007 Dec 28;282(52):37826-35. Epub 2007 Oct 31. PMID:17977832 Page seeded by OCA on Sun May 4 19:56:04 2008

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