2c1w
From Proteopedia
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| - | [[Image:2c1w.gif|left|200px]]<br /> | + | [[Image:2c1w.gif|left|200px]]<br /><applet load="2c1w" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2c1w" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2c1w, resolution 2.20Å" /> | caption="2c1w, resolution 2.20Å" /> | ||
'''THE STRUCTURE OF XENDOU: A SPLICING INDEPENDENT SNORNA PROCESSING ENDORIBONUCLEASE'''<br /> | '''THE STRUCTURE OF XENDOU: A SPLICING INDEPENDENT SNORNA PROCESSING ENDORIBONUCLEASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2C1W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 2C1W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Po4 Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C1W OCA]. |
==Reference== | ==Reference== | ||
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[[Category: splicing independent processing]] | [[Category: splicing independent processing]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:06:26 2007'' |
Revision as of 16:56, 18 December 2007
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THE STRUCTURE OF XENDOU: A SPLICING INDEPENDENT SNORNA PROCESSING ENDORIBONUCLEASE
Overview
Small nucleolar RNAs (snoRNAs) play a key role in eukaryotic ribosome, biogenesis. In most cases, snoRNAs are encoded in introns and are released, through the splicing reaction. Some snoRNAs are, instead, produced by an, alternative pathway consisting of endonucleolytic processing of pre-mRNA., XendoU, the endoribonuclease responsible for this activity, is a, U-specific, metal-dependent enzyme that releases products with 2'-3', cyclic phosphate termini. XendoU is broadly conserved among eukaryotes, and it is a genetic marker of nidoviruses, including the severe acute, respiratory syndrome coronavirus, where it is essential for replication, and transcription. We have determined by crystallography the structure of, XendoU that, by refined search methodologies, appears to display a unique, fold. Based on sequence conservation, mutagenesis, and docking, simulations, we have identified the active site. The conserved structural, determinants of this site may provide a framework for attempting to design, antiviral drugs to interfere with the infectious nidovirus life cycle.
About this Structure
2C1W is a Single protein structure of sequence from Xenopus laevis with PO4 as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The structure of the endoribonuclease XendoU: From small nucleolar RNA processing to severe acute respiratory syndrome coronavirus replication., Renzi F, Caffarelli E, Laneve P, Bozzoni I, Brunori M, Vallone B, Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12365-70. Epub 2006 Aug 8. PMID:16895992
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