2zak

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[[Image:2zak.jpg|left|200px]]
[[Image:2zak.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2zak |SIZE=350|CAPTION= <scene name='initialview01'>2zak</scene>, resolution 2.01&Aring;
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The line below this paragraph, containing "STRUCTURE_2zak", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Na+Binding+Site+For+Residue+A+322'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Residue+A+323'>AC2</scene>, <scene name='pdbsite=AC3:Na+Binding+Site+For+Residue+B+322'>AC3</scene>, <scene name='pdbsite=AC4:Cl+Binding+Site+For+Residue+B+323'>AC4</scene> and <scene name='pdbsite=AC5:Trs+Binding+Site+For+Residue+A+1'>AC5</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= ybiK (iaaA) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04701 Asparaginase_2]</span>
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{{STRUCTURE_2zak| PDB=2zak | SCENE= }}
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|RELATEDENTRY=[[1k2x|1K2X]], [[1jn9|1JN9]], [[1t3m|1T3M]], [[1seo|1SEO]], [[2gez|2GEZ]], [[2gac|2GAC]], [[9gac|9GAC]], [[9gaf|9GAF]], [[9gaa|9GAA]], [[1p4v|1P4V]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zak OCA], [http://www.ebi.ac.uk/pdbsum/2zak PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zak RCSB]</span>
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}}
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'''Orthorhombic crystal structure of precursor E. coli isoaspartyl peptidase/L-asparaginase (EcAIII) with active-site T179A mutation'''
'''Orthorhombic crystal structure of precursor E. coli isoaspartyl peptidase/L-asparaginase (EcAIII) with active-site T179A mutation'''
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[[Category: Jaskolski, M.]]
[[Category: Jaskolski, M.]]
[[Category: Michalska, K.]]
[[Category: Michalska, K.]]
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[[Category: asparaginase]]
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[[Category: Asparaginase]]
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[[Category: autoproteolysis]]
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[[Category: Autoproteolysis]]
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[[Category: isoaspartyl peptidase]]
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[[Category: Isoaspartyl peptidase]]
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[[Category: ntn-hydrolase]]
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[[Category: Ntn-hydrolase]]
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[[Category: precursor]]
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[[Category: Precursor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:05:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:19:55 2008''
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Revision as of 17:05, 4 May 2008

Image:2zak.jpg

Template:STRUCTURE 2zak

Orthorhombic crystal structure of precursor E. coli isoaspartyl peptidase/L-asparaginase (EcAIII) with active-site T179A mutation


Overview

Plant-type L-asparaginases hydrolyze the side-chain amide bond of L-asparagine or its beta-peptides. They belong to the N-terminal nucleophile (Ntn) hydrolases and are synthesized as inactive precursor molecules. Activation occurs via the autoproteolytic release of two subunits, alpha and beta, the latter of which carries the nucleophile at its N-terminus. Crystallographic studies of plant-type asparaginases have focused on an Escherichia coli homologue (EcAIII), which has been crystallized in several crystal forms. Although they all belong to the same P2(1)2(1)2(1) space group with similar unit-cell parameters, they display different crystal-packing arrangements and thus should be classified as separate polymorphs. This variability stems mainly from different positions of the EcAIII molecules within the unit cell, although they also exhibit slight differences in orientation. The intermolecular interactions that trigger different crystal lattice formation are mediated by ions, which represent the most variable component of the crystallization conditions. This behaviour confirms recent observations that small molecules might promote protein crystal lattice formation.

About this Structure

2ZAK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal packing of plant-type L-asparaginase from Escherichia coli., Michalska K, Borek D, Hernandez-Santoyo A, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2008 Mar;64(Pt 3):309-20. Epub 2008, Feb 20. PMID:18323626 Page seeded by OCA on Sun May 4 20:05:44 2008

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