2zai

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[[Image:2zai.jpg|left|200px]]
[[Image:2zai.jpg|left|200px]]
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{{Structure
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|PDB= 2zai |SIZE=350|CAPTION= <scene name='initialview01'>2zai</scene>, resolution 2.7&Aring;
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The line below this paragraph, containing "STRUCTURE_2zai", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>
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|GENE= PF0156 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus])
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|DOMAIN=
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{{STRUCTURE_2zai| PDB=2zai | SCENE= }}
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|RELATEDENTRY=[[2zag|2ZAG]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zai OCA], [http://www.ebi.ac.uk/pdbsum/2zai PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zai RCSB]</span>
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'''Crystal structure of the soluble domain of STT3 from P. furiosus'''
'''Crystal structure of the soluble domain of STT3 from P. furiosus'''
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Maita, N.]]
[[Category: Maita, N.]]
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[[Category: multi-domain proteins (alpha and beta)]]
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[[Category: Transferase]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:05:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:19:57 2008''
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Revision as of 17:05, 4 May 2008

Image:2zai.jpg

Template:STRUCTURE 2zai

Crystal structure of the soluble domain of STT3 from P. furiosus


Overview

Asn-glycosylation is widespread not only in eukaryotes but also in archaea and some eubacteria. Oligosaccharyltransferase (OST) catalyzes the co-translational transfer of an oligosaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains. Here, we report that a thermophilic archaeon, Pyrococcus furiosus OST is composed of the STT3 protein alone, and catalyzes the transfer of a heptasaccharide, containing one hexouronate and two pentose residues, onto peptides in an Asn-X-Thr/Ser-motif-dependent manner. We also determined the 2.7-A resolution crystal structure of the C-terminal soluble domain of Pyrococcus STT3. The structure-based multiple sequence alignment revealed a new motif, DxxK, which is adjacent to the well-conserved WWDYG motif in the tertiary structure. The mutagenesis of the DK motif residues in yeast STT3 revealed the essential role of the motif in the catalytic activity. The function of this motif may be related to the binding of the pyrophosphate group of lipid-linked oligosaccharide donors through a transiently bound cation. Our structure provides the first structural insights into the formation of the oligosaccharide-asparagine bond.

About this Structure

2ZAI is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.

Reference

Structure-guided identification of a new catalytic motif of oligosaccharyltransferase., Igura M, Maita N, Kamishikiryo J, Yamada M, Obita T, Maenaka K, Kohda D, EMBO J. 2008 Jan 9;27(1):234-43. Epub 2007 Nov 29. PMID:18046457 Page seeded by OCA on Sun May 4 20:05:36 2008

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