2zb9
From Proteopedia
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'''Crystal structure of TetR family transcription regulator SCO0332''' | '''Crystal structure of TetR family transcription regulator SCO0332''' | ||
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[[Category: Watanabe, N.]] | [[Category: Watanabe, N.]] | ||
[[Category: Yao, M.]] | [[Category: Yao, M.]] | ||
| - | [[Category: | + | [[Category: Dna-binding]] |
| - | [[Category: | + | [[Category: Helix-turn-helix]] |
| - | [[Category: | + | [[Category: Tetr family]] |
| - | [[Category: | + | [[Category: Transcription regulation]] |
| - | [[Category: | + | [[Category: Transcription regulator]] |
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Revision as of 17:06, 4 May 2008
Crystal structure of TetR family transcription regulator SCO0332
Overview
SCO0332 protein is a putative TetR-family transcriptional regulator from Streptomyces coelicolor A3(2). The crystal structure of SCO0332 was determined at 2.25 A resolution by single-wavelength anomalous diffraction (SAD) phasing using the S atoms of the native protein. SCO0332 contains a helix-turn-helix (HTH) DNA-binding motif in its N-terminal region and forms a homodimer. The overall structure of SCO0332 shows significant similarity to other TetR-family regulators. A systematic evolution of ligands by exponential enrichment (SELEX) analysis indicated that SCO0332 has sequence-specific DNA-binding ability and determined the position of the operator element of SCO0332 on the chromosomal DNA of S. coelicolor. An electrophoretic mobility-shift assay (EMSA) showed that SCO0332 binds to the operator sequence upstream of the sco0330 gene, which encodes a putative short-chain oxidoreductase. These results suggest that SCO0332 is a transcriptional repressor that regulates sco0330 gene expression.
About this Structure
2ZB9 is a Single protein structure of sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA.
Reference
Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor., Okada U, Kondo K, Hayashi T, Watanabe N, Yao M, Tamura T, Tanaka I, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):198-205. Epub 2008, Jan 16. PMID:18219120 Page seeded by OCA on Sun May 4 20:06:27 2008
