2zcf

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[[Image:2zcf.gif|left|200px]]
[[Image:2zcf.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2zcf |SIZE=350|CAPTION= <scene name='initialview01'>2zcf</scene>, resolution 1.43&Aring;
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The line below this paragraph, containing "STRUCTURE_2zcf", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= nthA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis]), nthB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis])
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-->
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|DOMAIN=
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{{STRUCTURE_2zcf| PDB=2zcf | SCENE= }}
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|RELATEDENTRY=[[1ahj|1AHJ]], [[2ahj|2AHJ]], [[2cyz|2CYZ]], [[2cz0|2CZ0]], [[2cz1|2CZ1]], [[2d0q|2D0Q]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zcf OCA], [http://www.ebi.ac.uk/pdbsum/2zcf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2zcf RCSB]</span>
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}}
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'''Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771'''
'''Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771'''
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[[Category: Ueda, S.]]
[[Category: Ueda, S.]]
[[Category: Yohda, M.]]
[[Category: Yohda, M.]]
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[[Category: cysteine-sulfenic acid]]
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[[Category: Cysteine-sulfenic acid]]
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[[Category: cysteine-sulfinic acid]]
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[[Category: Cysteine-sulfinic acid]]
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[[Category: hydration]]
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[[Category: Hydration]]
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[[Category: iron]]
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[[Category: Iron]]
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[[Category: lyase]]
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[[Category: Lyase]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: national project on protein structural and functional analyse]]
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[[Category: National project on protein structural and functional analyse]]
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[[Category: nitrile]]
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[[Category: Nitrile]]
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[[Category: nppsfa]]
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[[Category: Nppsfa]]
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[[Category: oxidation]]
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[[Category: Oxidation]]
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[[Category: photo-activation]]
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[[Category: Photo-activation]]
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[[Category: photo-reactive]]
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[[Category: Photo-reactive]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: Rsgi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:08:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:20:25 2008''
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Revision as of 17:08, 4 May 2008

Image:2zcf.gif

Template:STRUCTURE 2zcf

Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771


Overview

Nitrile hydratase (NHase) from Rhodococcus sp. N771 is a non-heme iron enzyme having post-translationally modified cysteine ligands, alphaCys112-SO2H and alphaCys114-SOH. We replaced alphaGln90, which is conserved in all known NHases and involved in the hydrogen-bond network around the catalytic center, with glutamic acid or asparagine. The kcat of alphaQ90E and alphaQ90N mutants decreased to 24% and 5% that of wild type respectively, but the effect of mutations on Km was not very significant. In both mutants, the alphaCys114-SOH modification appeared to be responsible for the catalysis as in native NHase. We crystallized the nitrosylated alphaQ90N mutant and determined its structure at a resolution of 1.43 A. The structure was basically identical to that of native nitrosylated NHase except for the mutated site and its vicinity. The structural difference between native and alphaQ90N mutant NHases suggested the importance of the hydrogen bond networks between alphaGln90 and the iron center for the catalytic activity.

About this Structure

2ZCF is a Protein complex structure of sequences from Rhodococcus erythropolis. Full crystallographic information is available from OCA.

Reference

Mutational study on alphaGln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771., Takarada H, Kawano Y, Hashimoto K, Nakayama H, Ueda S, Yohda M, Kamiya N, Dohmae N, Maeda M, Odaka M, Biosci Biotechnol Biochem. 2006 Apr;70(4):881-9. PMID:16636455 Page seeded by OCA on Sun May 4 20:08:29 2008

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