344d

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[[Image:344d.gif|left|200px]]
[[Image:344d.gif|left|200px]]
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{{Structure
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|PDB= 344d |SIZE=350|CAPTION= <scene name='initialview01'>344d</scene>, resolution 1.460&Aring;
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The line below this paragraph, containing "STRUCTURE_344d", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BRU:5-BROMO-2&#39;-DEOXYURIDINE-5&#39;-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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|GENE=
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{{STRUCTURE_344d| PDB=344d | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=344d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=344d OCA], [http://www.ebi.ac.uk/pdbsum/344d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=344d RCSB]</span>
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'''DETERMINATION BY MAD-DM OF THE STRUCTURE OF THE DNA DUPLEX D(ACGTACG(5-BRU))2 AT 1.46A AND 100K'''
'''DETERMINATION BY MAD-DM OF THE STRUCTURE OF THE DNA DUPLEX D(ACGTACG(5-BRU))2 AT 1.46A AND 100K'''
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==About this Structure==
==About this Structure==
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344D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=344D OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=344D OCA].
==Reference==
==Reference==
Determination by MAD-DM of the structure of the DNA duplex d[ACGTACG(5-BrU)]2 at 1.46 A and 100 K., Todd AK, Adams A, Powell HR, Wilcock DJ, Thorpe JH, Lausi A, Zanini F, Wakelin LP, Cardin CJ, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):729-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089302 10089302]
Determination by MAD-DM of the structure of the DNA duplex d[ACGTACG(5-BrU)]2 at 1.46 A and 100 K., Todd AK, Adams A, Powell HR, Wilcock DJ, Thorpe JH, Lausi A, Zanini F, Wakelin LP, Cardin CJ, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):729-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089302 10089302]
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[[Category: Protein complex]]
 
[[Category: Adams, A.]]
[[Category: Adams, A.]]
[[Category: Cardin, C J.]]
[[Category: Cardin, C J.]]
[[Category: Powell, H R.]]
[[Category: Powell, H R.]]
[[Category: Todd, A R.]]
[[Category: Todd, A R.]]
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[[Category: a-dna]]
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[[Category: A-dna]]
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[[Category: double helix]]
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[[Category: Double helix]]
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[[Category: modified]]
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[[Category: Modified]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:16:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:21:35 2008''
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Revision as of 17:16, 4 May 2008

Image:344d.gif

Template:STRUCTURE 344d

DETERMINATION BY MAD-DM OF THE STRUCTURE OF THE DNA DUPLEX D(ACGTACG(5-BRU))2 AT 1.46A AND 100K


Overview

A four-wavelength MAD experiment on a new brominated octanucleotide is reported here. d[ACGTACG(5-BrU)], C77H81BrN30O32P7, Mr (DNA) = 2235, tetragonal, P43212 (No. 96), a = 43.597, c = 26.268 A, V = 49927.5 A3, Z = 8, T = 100 K, R = 10.91% for 4312 reflections between 15.0 and 1.46 A resolution. The self-complementary brominated octanucleotide d[ACGTACG(5-BrU)]2 has been crystallized and data measured to 1.45 A at both 293 K and a second crystal flash frozen at 100 K. The latter data collection was carried out to the same resolution at the four wavelengths 0.9344, 0.9216, 0.9208 and 0.9003 A, around the Br K edge at 0.92 A and the structure determined from a map derived from a MAD data analysis using pseudo-MIR methodology, as implemented in the program MLPHARE. This is one of the first successful MAD phasing experiments carried out at Sincrotrone Elettra in Trieste, Italy. The structure was refined using the data measured at 0.9003 A, anisotropic temperature factors and the restrained least-squares refinement implemented in the program SHELX96, and the helical parameters are compared with those previously determined for the isomorphous d(ACGTACGT)2 analogue. The asymmetric unit consists of a single strand of octamer with 96 water molecules. No countercations were located. The A-DNA helix geometry obtained has been analysed using the CURVES program.

About this Structure

Full crystallographic information is available from OCA.

Reference

Determination by MAD-DM of the structure of the DNA duplex d[ACGTACG(5-BrU)]2 at 1.46 A and 100 K., Todd AK, Adams A, Powell HR, Wilcock DJ, Thorpe JH, Lausi A, Zanini F, Wakelin LP, Cardin CJ, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):729-35. PMID:10089302 Page seeded by OCA on Sun May 4 20:16:38 2008

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