3fxe

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of interacting domains of IcmR and IcmQ (seleno-derivative)

Structural highlights

3fxe is a 2 chain structure with sequence from Legpc and Legph. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	icmQ, LPC_2899 (LEGPC), icmR, lpg0443 (LEGPH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

During infection, Legionella pneumophila creates a replication vacuole within eukaryotic cells and this requires a Type IVb secretion system (T4bSS). IcmQ plays a critical role in the translocase and associates with IcmR. In this paper, we show that the N-terminal domain of IcmQ (Qn) mediates self-dimerization, whereas the C-terminal domain with a basic linker promotes membrane association. In addition, the binding of IcmR to IcmQ prevents self-dimerization and also blocks membrane permeabilization. However, IcmR does not completely block membrane binding by IcmQ. We then determined crystal structures of Qn with the interacting region of IcmR. In this complex, each protein forms an alpha-helical hairpin within a parallel four-helix bundle. The amphipathic nature of helices in Qn suggests two possible models for membrane permeabilization by IcmQ. The Rm-Qn structure also suggests how IcmR-like proteins in other L. pneumophila species may interact with their IcmQ partners.

Structure and function of interacting IcmR-IcmQ domains from a type IVb secretion system in Legionella pneumophila.,Raychaudhury S, Farelli JD, Montminy TP, Matthews M, Menetret JF, Dumenil G, Roy CR, Head JF, Isberg RR, Akey CW Structure. 2009 Apr 15;17(4):590-601. PMID:19368892^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Raychaudhury S, Farelli JD, Montminy TP, Matthews M, Menetret JF, Dumenil G, Roy CR, Head JF, Isberg RR, Akey CW. Structure and function of interacting IcmR-IcmQ domains from a type IVb secretion system in Legionella pneumophila. Structure. 2009 Apr 15;17(4):590-601. PMID:19368892 doi:10.1016/j.str.2009.02.011

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3fxe"

@@ Line 1: / Line 1: @@
 ==Crystal structure of interacting domains of IcmR and IcmQ (seleno-derivative)==
-<StructureSection load='3fxe' size='340' side='right' caption='[[3fxe]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='3fxe' size='340' side='right'caption='[[3fxe]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3fxe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legpc Legpc] and [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FXE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FXE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3fxe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legpc Legpc] and [https://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FXE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FXE FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">icmQ, LPC_2899 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=400673 LEGPC]), icmR, lpg0443 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">icmQ, LPC_2899 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=400673 LEGPC]), icmR, lpg0443 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fxe OCA], [http://pdbe.org/3fxe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fxe RCSB], [http://www.ebi.ac.uk/pdbsum/3fxe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fxe ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fxe OCA], [https://pdbe.org/3fxe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fxe RCSB], [https://www.ebi.ac.uk/pdbsum/3fxe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fxe ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 31: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Legpc]]
 [[Category: Legph]]

3fxe

From Proteopedia

Current revision

Crystal structure of interacting domains of IcmR and IcmQ (seleno-derivative)

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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