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6ifl
From Proteopedia
(Difference between revisions)
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<StructureSection load='6ifl' size='340' side='right' caption='[[6ifl]], [[Resolution|resolution]] 3.16Å' scene=''> | <StructureSection load='6ifl' size='340' side='right' caption='[[6ifl]], [[Resolution|resolution]] 3.16Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6ifl]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IFL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ifl]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19258 Atcc 19258] and [http://en.wikipedia.org/wiki/Strtn Strtn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IFL FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ifl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ifl OCA], [http://pdbe.org/6ifl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ifl RCSB], [http://www.ebi.ac.uk/pdbsum/6ifl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ifl ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=767463 STRTN]), csm2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=767463 STRTN]), csm3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=767463 STRTN]), csm4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=767463 STRTN]), csm5, DID82_08845 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1308 ATCC 19258])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ifl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ifl OCA], [http://pdbe.org/6ifl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ifl RCSB], [http://www.ebi.ac.uk/pdbsum/6ifl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ifl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Csm, a type III-A CRISPR-Cas interference complex, is a CRISPR RNA (crRNA)-guided RNase that also possesses target RNA-dependent DNase and cyclic oligoadenylate (cOA) synthetase activities. However, the structural features allowing target RNA-binding-dependent activation of DNA cleavage and cOA generation remain unknown. Here, we report the structure of Csm in complex with crRNA together with structures of cognate or non-cognate target RNA bound Csm complexes. We show that depending on complementarity with the 5' tag of crRNA, the 3' anti-tag region of target RNA binds at two distinct sites of the Csm complex. Importantly, the interaction between the non-complementary anti-tag region of cognate target RNA and Csm1 induces a conformational change at the Csm1 subunit that allosterically activates DNA cleavage and cOA generation. Together, our structural studies provide crucial insights into the mechanistic processes required for crRNA-meditated sequence-specific RNA cleavage, RNA target-dependent non-specific DNA cleavage, and cOA generation. | ||
| + | |||
| + | Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference.,You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y Cell. 2018 Nov 26. pii: S0092-8674(18)31451-X. doi: 10.1016/j.cell.2018.10.052. PMID:30503210<ref>PMID:30503210</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6ifl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Atcc 19258]] | ||
| + | [[Category: Strtn]] | ||
[[Category: Ma, J]] | [[Category: Ma, J]] | ||
[[Category: Wang, J]] | [[Category: Wang, J]] | ||
Revision as of 09:03, 19 December 2018
Cryo-EM structure of type III-A Csm-NTR complex
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Categories: Atcc 19258 | Strtn | Ma, J | Wang, J | Wang, Y | You, L | Zhang, X | Crispr-cas system | Csm complex | Rna binding protein | Type iii-a
