6mur
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
<StructureSection load='6mur' size='340' side='right' caption='[[6mur]], [[Resolution|resolution]] 3.10Å' scene=''> | <StructureSection load='6mur' size='340' side='right' caption='[[6mur]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6mur]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MUR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mur]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermococcus_onnurineus"_bae_et_al._2006 "thermococcus onnurineus" bae et al. 2006] and [http://en.wikipedia.org/wiki/Theon Theon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MUR FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6mua|6mua]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6mua|6mua]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TON_0893 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=523850 THEON]), TON_0894 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=523850 THEON]), TON_0895 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342948 "Thermococcus onnurineus" Bae et al. 2006]), TON_0896 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=523850 THEON]), TON_0897 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=523850 THEON])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mur OCA], [http://pdbe.org/6mur PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mur RCSB], [http://www.ebi.ac.uk/pdbsum/6mur PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mur ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mur OCA], [http://pdbe.org/6mur PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mur RCSB], [http://www.ebi.ac.uk/pdbsum/6mur PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mur ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Type IotaIotaIota CRISPR-Cas systems provide robust immunity against foreign RNA and DNA by sequence-specific RNase and target RNA-activated sequence-nonspecific DNase and RNase activities. We report on cryo-EM structures of Thermococcus onnurineus Csm(crRNA) binary, Csm(crRNA)-target RNA and Csm(crRNA)-target RNA(anti-tag) ternary complexes in the 3.1 A range. The topological features of the crRNA 5'-repeat tag explains the 5'-ruler mechanism for defining target cleavage sites, with accessibility of positions -2 to -5 within the 5'-repeat serving as sensors for avoidance of autoimmunity. The Csm3 thumb elements introduce periodic kinks in the crRNA-target RNA duplex, facilitating cleavage of the target RNA with 6-nt periodicity. Key Glu residues within a Csm1 loop segment of Csm(crRNA) adopt a proposed autoinhibitory conformation suggestive of DNase activity regulation. These structural findings, complemented by mutational studies of key intermolecular contacts, provide insights into Csm(crRNA) complex assembly, mechanisms underlying RNA targeting and site-specific periodic cleavage, regulation of DNase cleavage activity, and autoimmunity suppression. | ||
| + | |||
| + | Type III-A CRISPR-Cas Csm Complexes: Assembly, Periodic RNA Cleavage, DNase Activity Regulation, and Autoimmunity.,Jia N, Mo CY, Wang C, Eng ET, Marraffini LA, Patel DJ Mol Cell. 2018 Nov 27. pii: S1097-2765(18)30977-8. doi:, 10.1016/j.molcel.2018.11.007. PMID:30503773<ref>PMID:30503773</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6mur" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Thermococcus onnurineus bae et al. 2006]] | ||
| + | [[Category: Theon]] | ||
[[Category: Eng, E T]] | [[Category: Eng, E T]] | ||
[[Category: Jia, N]] | [[Category: Jia, N]] | ||
Revision as of 09:08, 19 December 2018
Cryo-EM structure of Csm-crRNA-target RNA ternary complex in type III-A CRISPR-Cas system
| |||||||||||
