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1c03

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Revision as of 10:31, 16 September 2020

CRYSTAL STRUCTURE OF YPD1P (TRICLINIC FORM)

Structural highlights

1c03 is a 4 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2a0b
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[YPD1_YEAST] Phosphorelay intermediate protein that is part of the branched SLN1-YPD1-SKN7/SSK1 two-component regulatory system, which controls activity of the HOG1 pathway and gene expression in response to changes in the osmolarity of the extracellular environment. Catalyzes the phosphoryl group transfer from the membrane-bound osmosensing histidine kinase SLN1 to two distinct response regulator proteins, SSK1 in the cytoplasm, and transcription factor SKN7 in the nucleus.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

"Two-component" phosphorelay signal transduction systems constitute a potential target for antibacterial and antifungal agents, since they are found exclusively in prokaryotes and lower eukaryotes (yeast, fungi, slime mold, and plants) but not in mammalian organisms. Saccharomyces cerevisiae Ypd1p, a key intermediate in the osmosensing multistep phosphorelay signal transduction, catalyzes the phosphoryl group transfer between response regulators. Its 1.8 A structure, representing the first example of a eukaryotic phosphorelay protein, contains a four-helix bundle as in the HPt domain of Escherichia coli ArcB sensor kinase. However, Ypd1p has a 44-residue insertion between the last two helices of the helix bundle. The side-chain of His64, the site of phosphorylation, protrudes into the solvent. The structural resemblance between Ypd1p and ArcB HPt domain suggests that both prokaryotes and lower eukaryotes utilize the same basic protein fold for phosphorelay signal transduction. This study sheds light on the best characterized eukaryotic phosphorelay system.

Insights into eukaryotic multistep phosphorelay signal transduction revealed by the crystal structure of Ypd1p from Saccharomyces cerevisiae.,Song HK, Lee JY, Lee MG, Moon J, Min K, Yang JK, Suh SW J Mol Biol. 1999 Nov 5;293(4):753-61. PMID:10543964^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Janiak-Spens F, Sparling DP, West AH. Novel role for an HPt domain in stabilizing the phosphorylated state of a response regulator domain. J Bacteriol. 2000 Dec;182(23):6673-8. PMID:11073911
↑ Li S, Ault A, Malone CL, Raitt D, Dean S, Johnston LH, Deschenes RJ, Fassler JS. The yeast histidine protein kinase, Sln1p, mediates phosphotransfer to two response regulators, Ssk1p and Skn7p. EMBO J. 1998 Dec 1;17(23):6952-62. PMID:9843501 doi:http://dx.doi.org/10.1093/emboj/17.23.6952
↑ Song HK, Lee JY, Lee MG, Moon J, Min K, Yang JK, Suh SW. Insights into eukaryotic multistep phosphorelay signal transduction revealed by the crystal structure of Ypd1p from Saccharomyces cerevisiae. J Mol Biol. 1999 Nov 5;293(4):753-61. PMID:10543964 doi:10.1006/jmbi.1999.3215

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1c03"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF YPD1P (TRICLINIC FORM)==
-<StructureSection load='1c03' size='340' side='right' caption='[[1c03]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='1c03' size='340' side='right'caption='[[1c03]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1c03]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C03 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C03 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1c03]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C03 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1C03 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a0b|2a0b]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c03 OCA], [http://pdbe.org/1c03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c03 RCSB], [http://www.ebi.ac.uk/pdbsum/1c03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1c03 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1c03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c03 OCA], [http://pdbe.org/1c03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c03 RCSB], [http://www.ebi.ac.uk/pdbsum/1c03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1c03 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 37: / Line 37: @@
 </StructureSection>
 [[Category: Atcc 18824]]
+[[Category: Large Structures]]
 [[Category: Lee, J Y]]
 [[Category: Lee, M G]]

1c03

From Proteopedia

Revision as of 10:31, 16 September 2020

CRYSTAL STRUCTURE OF YPD1P (TRICLINIC FORM)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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