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Actin

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<scene name='43/430015/Cv/10'>Actin binds ATP</scene> in a cleft. Water molecules are shown as red spheres. <scene name='43/430015/Cv/13'>ATP and Ca2+ ion are located in cleft</scene>. <scene name='43/430015/Cv/11'>Click here to see Ca2+ ion coordination site</scene>.<ref>PMID:20540085</ref> It changes its conformation upon hydrolysis of its bound ATP to ADP. Actin filaments are polar. They are formed with all monomers having their clefts pointing in the same direction.
<scene name='43/430015/Cv/10'>Actin binds ATP</scene> in a cleft. Water molecules are shown as red spheres. <scene name='43/430015/Cv/13'>ATP and Ca2+ ion are located in cleft</scene>. <scene name='43/430015/Cv/11'>Click here to see Ca2+ ion coordination site</scene>.<ref>PMID:20540085</ref> It changes its conformation upon hydrolysis of its bound ATP to ADP. Actin filaments are polar. They are formed with all monomers having their clefts pointing in the same direction.
 +
 +
== 3D Structures of Actin ==
 +
[[Actin 3D structures]]
 +
</StructureSection>
</StructureSection>
== 3D Structures of Actin ==
== 3D Structures of Actin ==
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**[[2hmp]] – rAa – protease cleaved<br />
**[[2hmp]] – rAa – protease cleaved<br />
**[[2zwh]], [[2gwj]], [[2gwk]] – rAa-ATP<br />
**[[2zwh]], [[2gwj]], [[2gwk]] – rAa-ATP<br />
-
**[[3j8i]], [[3j8j]], [[3j8k]], [[6bno]], [[6avb]], [[6av9]], [[5mvy]], [[5mva]] – rAa + ADP – Cryo EM<br />
+
**[[3j8i]], [[3j8j]], [[3j8k]], [[6bno]], [[6avb]], [[6av9]], [[5mvy]], [[5mva]], [[6fhl]], [[5oof]], [[5ooe]], [[5ood]], [[5ooc]], [[5onv]] – rAa + ADP – Cryo EM<br />
**[[3b5u]] – rAa filament – fiber diffraction<br />
**[[3b5u]] – rAa filament – fiber diffraction<br />
Line 42: Line 46:
* α actin 1 complex with small molecule
* α actin 1 complex with small molecule
-
**[[2q36]], [[1qz5]] – rAa+kabiramide C + ATP – dimer<br />
+
**[[2q36]], [[1qz5]] – rAa+kabiramide C + ATP <br />
**[[2vyp]] – rAa+myxobacterial rhizopodin + ATP<br />
**[[2vyp]] – rAa+myxobacterial rhizopodin + ATP<br />
**[[1ijj]] - rAa+latrunculin A<br />
**[[1ijj]] - rAa+latrunculin A<br />
**[[2fxu]] – rAa+bistramide A<br />
**[[2fxu]] – rAa+bistramide A<br />
**[[1wua]] – rAa+alpyronine A<br />
**[[1wua]] – rAa+alpyronine A<br />
-
**[[2asm]], [[2asp]] – rAa+reidispongiolide<br />
+
**[[4k41]], [[1qz6]], [[1s22]], [[4k42]], [[4k43]], [[2asm]], [[2asp]], [[2aso]], [[1yxq]], [[1s22]], [[3m6g]], [[6mgo]] - rAa+ macrolide<br />
-
**[[2aso]] – rAa+sphinxolide B<br />
+
-
**[[1yxq]] – rAa+swinholide A<br />
+
-
**[[4k41]], [[1qz6]], [[1s22]], [[4k42]], [[4k43]] - rAa+ macrolide<br />
+
-
**[[1s22]] – rAa+ulapualide A<br />
+
**[[1qz6]] – rAa+jaspisamide A<br />
**[[1qz6]] – rAa+jaspisamide A<br />
-
**[[3m6g]] – rAa+lobophorolide<br />
 
**[[5ogw]] – PfAa + jasplakinolide + ADP – Cryo EM <br />
**[[5ogw]] – PfAa + jasplakinolide + ADP – Cryo EM <br />
 +
**[[6bqw]] – Aa + AMPPNP – ''Bacillus subtilis'' - Cryo EM <br />
* α actin 1 complex with protein
* α actin 1 complex with protein
 +
**[[6fm2]] – hAa + adenylyl cyclase-asociated protein <br />
**[[2w49]] – cAa +troponin C+ troponin T+ troponin I+ tropomyosin α-1 <br />
**[[2w49]] – cAa +troponin C+ troponin T+ troponin I+ tropomyosin α-1 <br />
**[[3j4k]] - cAa + tropomyosin<br />
**[[3j4k]] - cAa + tropomyosin<br />
 +
**[[6d8c]] - cAa + filamin + phalloidin – Cryo EM<br />
 +
**[[5yu8]] – cAa + cofilin 2<br />
**[[3j8a]] - mAa + tropomyosin - mouse<br />
**[[3j8a]] - mAa + tropomyosin - mouse<br />
**[[4a7f]], [[4a7h]], [[4a7l]], [[5h53]], [[5kg8]] – rAa + tropomyosin α-1 + myosin heavy chain (mutant) – Cryo EM<br />
**[[4a7f]], [[4a7h]], [[4a7l]], [[5h53]], [[5kg8]] – rAa + tropomyosin α-1 + myosin heavy chain (mutant) – Cryo EM<br />
-
**[[5nol]], [[5noj]], [[5nog]] – Aa + tropomyosin alpha-1 + ADP – pig - Cryo EM<br />
 
**[[5jlf]], [[3j8a]] – rAa + tropomyosin alpha-1 + ADP – Cryo EM<br />
**[[5jlf]], [[3j8a]] – rAa + tropomyosin alpha-1 + ADP – Cryo EM<br />
**[[6c1h]], [[6c1g]], [[6c1d]] - rAa + myosin + calmodulin<br />
**[[6c1h]], [[6c1g]], [[6c1d]] - rAa + myosin + calmodulin<br />
**[[6bnv]] - rAa + myosin + calmodulin – Cryo EM<br />
**[[6bnv]] - rAa + myosin + calmodulin – Cryo EM<br />
**[[6bnw]], [[6bnp]] - rAa + myosin – Cryo EM<br />
**[[6bnw]], [[6bnp]] - rAa + myosin – Cryo EM<br />
-
**[[6bnq]] - rAa + myosin + ADP – Cryo EM<br />
+
**[[6bnq]], [[6blh]] - rAa + myosin + ADP – Cryo EM<br />
**[[2v51]], [[2v52]], [[2yje]], [[2yjf]] – rAa+mMKL/Myocardin-like protein <br />
**[[2v51]], [[2v52]], [[2yje]], [[2yjf]] – rAa+mMKL/Myocardin-like protein <br />
**[[2vcp]] – rAa+ neural wiskott-aldrich syndrome protein (hWASP) – human (h)<br />
**[[2vcp]] – rAa+ neural wiskott-aldrich syndrome protein (hWASP) – human (h)<br />
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**[[3cjc]] - rAa+hGelsolin+cDNase I<br />
**[[3cjc]] - rAa+hGelsolin+cDNase I<br />
**[[2d1k]] - rAa+hWH2 domain of MIN+cDNase I<br />
**[[2d1k]] - rAa+hWH2 domain of MIN+cDNase I<br />
-
**[[2pbd]] – rAa+hProfilin-1<br />
+
**[[2pbd]], [[5zzb]], [[5zza]], [[5yee]] – rAa+hProfilin-1<br />
**[[2pav]] - rAa+hProfilin-1+hVASP<br />
**[[2pav]] - rAa+hProfilin-1+hVASP<br />
**[[2q97]] – rAa+toxofilin – ''Toxoplasma gondii''<br />
**[[2q97]] – rAa+toxofilin – ''Toxoplasma gondii''<br />
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**[[4h0t]] – rAa + ι toxin component IA + ADPR<br />
**[[4h0t]] – rAa + ι toxin component IA + ADPR<br />
**[[4h03]], [[4h0v]], [[4h0x]], [[4h0y]] – rAa + ι toxin component IA + NAD<br />
**[[4h03]], [[4h0v]], [[4h0x]], [[4h0y]] – rAa + ι toxin component IA + NAD<br />
-
**[[4jhd]] – DmAa + protein cordon-bleu WH2 domain<br />
+
**[[5ypu]] – rAa + protein cordon-bleu WH2 domain<br />
**[[5i9e]] – yAa + actin-related protein 4 + helicase <br />
**[[5i9e]] – yAa + actin-related protein 4 + helicase <br />
**[[5ce3]], [[4ci6]] – Aa + protein kinase YopO – fall armyworm <br />
**[[5ce3]], [[4ci6]] – Aa + protein kinase YopO – fall armyworm <br />
 +
**[[5nol]], [[5noj]], [[5nog]] – Aa + tropomyosin alpha-1 + ADP – pig - Cryo EM<br />
* β actin (Ab)
* β actin (Ab)
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* γ 1 actin (Ag)
* γ 1 actin (Ag)
 +
**[[1h1v]] – hAg+rGelsolin<br />
 +
**[[5jlh]], [[6g2t]], [[6cxj]], [[6cxi]] – hAg + myosin-14 + tropomyosin alpha-3 – Cryo-EM <br />
**[[1atn]] – rAg+cDNase I<br />
**[[1atn]] – rAg+cDNase I<br />
**[[3chw]] – Major DdAg+hProfilin+poly-Pro repeat – ''Dictyostelium discoideum''<br />
**[[3chw]] – Major DdAg+hProfilin+poly-Pro repeat – ''Dictyostelium discoideum''<br />
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**[[1nm1]] - DdAg+hGelsolin+Mg-ATP<br />
**[[1nm1]] - DdAg+hGelsolin+Mg-ATP<br />
**[[1nlv]], [[1c0f]] – DdAg+hGelsolin<br />
**[[1nlv]], [[1c0f]] – DdAg+hGelsolin<br />
-
**[[1d4x]] – Ag 1/3+hGelsolin – ''C. elegans''<br />
 
-
**[[1h1v]] – hAg+rGelsolin<br />
 
-
**[[5jlh]] – hAg + myosin-14 + tropomyosin alpha-3 – Cryo-EM <br />
 
**[[1hlu]], [[2btf]] – cAg+cProfilin<br />
**[[1hlu]], [[2btf]] – cAg+cProfilin<br />
**[[1o1g]], [[1o1a]], [[1o1b]], [[1o1c]], [[1o1d]], [[1o1e]], [[1o1f]], [[1o18]], [[1o19]], [[1mvw]], [[1m8q]] –cAg+cMyosin II - tomography<br />
**[[1o1g]], [[1o1a]], [[1o1b]], [[1o1c]], [[1o1d]], [[1o1e]], [[1o1f]], [[1o18]], [[1o19]], [[1mvw]], [[1m8q]] –cAg+cMyosin II - tomography<br />
-
**[[3eks]] – DmAg 5c (mutant)<br />
 
-
**[[3eku]] – DmAg 5c (mutant)+cytochalasin D<br />
 
-
**[[3el2]], [[2hf4]] - DmAg 5c (mutant)+Ca-ATP<br />
 
-
**[[2hf3]] - DmAg 5c (mutant)+Ca-ADP<br />
 
**[[3w3d]] – cAg + DNase I<br />
**[[3w3d]] – cAg + DNase I<br />
-
**[[3b63]] – actin filament – ''Limulus polyphemus''<br />
 
**[[1yvn]] – yA (mutant)+hGelsolin <br />
**[[1yvn]] – yA (mutant)+hGelsolin <br />
**[[1yag]] - yA+hGelsolin<br />
**[[1yag]] - yA+hGelsolin<br />
-
**[[3mmv]] – DmA-5C+spire WH2 domain<br />
+
**[[1d4x]] – Ag 1/3+hGelsolin – ''C. elegans''<br />
-
**[[3mn6]], [[3mn7]], [[3mn9]] - DmA-5C (mutant)+spire<br />
+
**[[3b63]] – actin filament – ''Limulus polyphemus''<br />
* Actin 5C (A5C)
* Actin 5C (A5C)
**[[4rwt]], [[5wfn]] – DmA5C + leiomodin<br />
**[[4rwt]], [[5wfn]] – DmA5C + leiomodin<br />
-
 
+
**[[3eks]] – DmAg 5c (mutant)<br />
 +
**[[3eku]] – DmAg 5c (mutant)+cytochalasin D<br />
 +
**[[3el2]], [[2hf4]] - DmAg 5c (mutant)+Ca-ATP<br />
 +
**[[2hf3]] - DmAg 5c (mutant)+Ca-ADP<br />
 +
**[[3mmv]] – DmA-5C+spire WH2 domain<br />
 +
**[[3mn6]], [[3mn7]], [[3mn9]] - DmA-5C (mutant)+spire<br />
 +
**[[4jhd]] – DmAg 5c + protein cordon-bleu WH2 domain<br />
**[[4efh]] – A + spire domains C,D – ''Acanthamoeba castellanii''
**[[4efh]] – A + spire domains C,D – ''Acanthamoeba castellanii''
}}
}}

Revision as of 09:45, 27 February 2019

Native G-actin with methyl-histidine complex with ATP, sulfate and Ca+2 ion (green) 3hbt

Drag the structure with the mouse to rotate

3D Structures of Actin

Updated on 27-February-2019

Reference

  1. ↑ Otterbein LR, Graceffa P, Dominguez R. The crystal structure of uncomplexed actin in the ADP state. Science. 2001 Jul 27;293(5530):708-11. PMID:11474115 doi:10.1126/science.1059700
  2. ↑ Wang H, Robinson RC, Burtnick LD. The structure of native G-actin. Cytoskeleton (Hoboken). 2010 Jul;67(7):456-65. PMID:20540085 doi:10.1002/cm.20458

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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