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3bq3
From Proteopedia
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[[Image:3bq3.jpg|left|200px]] | [[Image:3bq3.jpg|left|200px]] | ||
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'''Crystal structure of S. cerevisiae Dcn1''' | '''Crystal structure of S. cerevisiae Dcn1''' | ||
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[[Category: Chou, Y C.]] | [[Category: Chou, Y C.]] | ||
[[Category: Sicheri, F.]] | [[Category: Sicheri, F.]] | ||
| - | [[Category: | + | [[Category: Cell cycle]] |
| - | [[Category: | + | [[Category: E2]] |
| - | [[Category: | + | [[Category: E3 ligase]] |
| - | [[Category: | + | [[Category: Ligase]] |
| - | [[Category: | + | [[Category: Nedd8]] |
| - | [[Category: | + | [[Category: Neddylation]] |
| - | [[Category: | + | [[Category: Protein degradation]] |
| - | [[Category: | + | [[Category: Ubiquitin]] |
| - | [[Category: | + | [[Category: Ubiquitination,scf,cullin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:00:02 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:00, 4 May 2008
Crystal structure of S. cerevisiae Dcn1
Overview
Cullin-based E3 ubiquitin ligases are activated through modification of the cullin subunit with the ubiquitin-like protein Nedd8. Dcn1 regulates cullin neddylation and thus ubiquitin ligase activity. Here we describe the 1.9 A X-ray crystal structure of yeast Dcn1 encompassing an N-terminal ubiquitin-binding (UBA) domain and a C-terminal domain of unique architecture, which we termed PONY domain. A conserved surface on Dcn1 is required for direct binding to cullins and for neddylation. The reciprocal binding site for Dcn1 on Cdc53 is located approximately 18 A from the site of neddylation. Dcn1 does not require cysteine residues for catalytic function, and directly interacts with the Nedd8 E2 Ubc12 on a surface that overlaps with the E1-binding site. We show that Dcn1 is necessary and sufficient for cullin neddylation in a purified recombinant system. Taken together, these data demonstrate that Dcn1 is a scaffold-like E3 ligase for cullin neddylation.
About this Structure
3BQ3 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Dcn1 functions as a scaffold-type E3 ligase for cullin neddylation., Kurz T, Chou YC, Willems AR, Meyer-Schaller N, Hecht ML, Tyers M, Peter M, Sicheri F, Mol Cell. 2008 Jan 18;29(1):23-35. PMID:18206966 Page seeded by OCA on Sun May 4 21:00:02 2008
