5zz9

From Proteopedia

(Difference between revisions)

Revision as of 21:25, 28 October 2020

Crystal structure of Homer2 EVH1/Drebrin PPXXF complex

Structural highlights

5zz9 is a 6 chain structure with sequence from Human and Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	Homer2, Vesl2 (LK3 transgenic mice), DBN1, D0S117E (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HOME2_MOUSE] Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses. [DREB_HUMAN] Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites. Required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS.^[1]

Publication Abstract from PubMed

Drebrin is an actin bundling protein that plays critical roles in synaptic spine development and plasticity. Homer, one of the most abundant scaffolding proteins in postsynaptic density, interacts with Drebrin's C-terminal PPXXF motifs using its Ena/VASP homology 1 (EVH1) domain. However, the molecular mechanism and biological function of this interaction remain unclear. Here we show that Homer specifically binds to the first but not the second PPXXF motif in Drebrin. The crystal structure of Drebrin-Homer binding motif 1 in complex with Homer EVH1 reveals a consensus Homer EVH1 binding motif. Homer tetramer promotes actin bundling activity of Drebrin in vitro and stimulates Drebrin-induced filopodia formation and elongation in cells. We further show that monomeric Homer1a antagonizes Homer1b in promoting Drebrin-stimulated actin bundling. Our study suggests a potential regulatory role of Homer1 in modulating excitatory synaptic spine homeostatic scaling via binding to Drebrin.

Homer Tetramer Promotes Actin Bundling Activity of Drebrin.,Li Z, Liu H, Li J, Yang Q, Feng Z, Li Y, Yang H, Yu C, Wan J, Liu W, Zhang M Structure. 2018 Nov 5. pii: S0969-2126(18)30372-1. doi:, 10.1016/j.str.2018.10.011. PMID:30503778^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Perez-Martinez M, Gordon-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vazquez J, Gonzalez-Amaro R, Veiga E, Sanchez-Madrid F. F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. J Cell Sci. 2010 Apr 1;123(Pt 7):1160-70. doi: 10.1242/jcs.064238. Epub 2010 Mar , 9. PMID:20215400 doi:http://dx.doi.org/10.1242/jcs.064238
↑ Li Z, Liu H, Li J, Yang Q, Feng Z, Li Y, Yang H, Yu C, Wan J, Liu W, Zhang M. Homer Tetramer Promotes Actin Bundling Activity of Drebrin. Structure. 2018 Nov 5. pii: S0969-2126(18)30372-1. doi:, 10.1016/j.str.2018.10.011. PMID:30503778 doi:http://dx.doi.org/10.1016/j.str.2018.10.011

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5zz9"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Homer2 EVH1/Drebrin PPXXF complex==
-<StructureSection load='5zz9' size='340' side='right' caption='[[5zz9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='5zz9' size='340' side='right'caption='[[5zz9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5zz9]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZZ9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5zz9]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZZ9 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zz9 OCA], [http://pdbe.org/5zz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zz9 RCSB], [http://www.ebi.ac.uk/pdbsum/5zz9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zz9 ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Homer2, Vesl2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), DBN1, D0S117E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zz9 OCA], [http://pdbe.org/5zz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zz9 RCSB], [http://www.ebi.ac.uk/pdbsum/5zz9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zz9 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
+[[Category: Lk3 transgenic mice]]
 [[Category: Li, J]]
 [[Category: Li, Z]]

5zz9

From Proteopedia

Revision as of 21:25, 28 October 2020

Crystal structure of Homer2 EVH1/Drebrin PPXXF complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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