3bum

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:3bum.jpg|left|200px]]
[[Image:3bum.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 3bum |SIZE=350|CAPTION= <scene name='initialview01'>3bum</scene>, resolution 2.000&Aring;
+
The line below this paragraph, containing "STRUCTURE_3bum", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= SPRY2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CBL, CBL2, RNF55 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_3bum| PDB=3bum | SCENE= }}
-
|RELATEDENTRY=[[3bun|3BUN]], [[3buo|3BUO]], [[3buw|3BUW]], [[3bux|3BUX]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bum OCA], [http://www.ebi.ac.uk/pdbsum/3bum PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bum RCSB]</span>
+
-
}}
+
'''Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty2'''
'''Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty2'''
Line 31: Line 28:
[[Category: Sivaraman, J.]]
[[Category: Sivaraman, J.]]
[[Category: Sun, Q.]]
[[Category: Sun, Q.]]
-
[[Category: calcium]]
+
[[Category: Calcium]]
-
[[Category: complex]]
+
[[Category: Complex]]
-
[[Category: cytoplasm]]
+
[[Category: Cytoplasm]]
-
[[Category: developmental protein]]
+
[[Category: Developmental protein]]
-
[[Category: ligase]]
+
[[Category: Ligase]]
-
[[Category: membrane]]
+
[[Category: Membrane]]
-
[[Category: metal-binding]]
+
[[Category: Metal-binding]]
-
[[Category: microtubule]]
+
[[Category: Microtubule]]
-
[[Category: phosphoprotein]]
+
[[Category: Phosphoprotein]]
-
[[Category: polymorphism]]
+
[[Category: Polymorphism]]
-
[[Category: proto-oncogene]]
+
[[Category: Proto-oncogene]]
-
[[Category: sh2 domain]]
+
[[Category: Sh2 domain]]
-
[[Category: signal transduction]]
+
[[Category: Signal transduction]]
-
[[Category: ubl conjugation pathway]]
+
[[Category: Ubl conjugation pathway]]
-
[[Category: zinc]]
+
[[Category: Zinc]]
-
[[Category: zinc-finger]]
+
[[Category: Zinc-finger]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:07:17 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:29:09 2008''
+

Revision as of 18:07, 4 May 2008

Image:3bum.jpg

Template:STRUCTURE 3bum

Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty2


Overview

The c-Cbl tyrosine kinase binding domain (Cbl-TKB), essentially an 'embedded' SH2 domain, has a critical role in targeting proteins for ubiquitination. To address how this domain can bind to disparate recognition mofits and to determine whether this results in variations in substrate-binding affinity, we compared crystal structures of the Cbl-TKB domain complexed with phosphorylated peptides of Sprouty2, Sprouty4, epidermal growth factor receptor, Syk, and c-Met receptors and validated the binding with point-mutational analyses using full-length proteins. An obligatory, intrapeptidyl H-bond between the phosphotyrosine and the conserved asparagine or adjacent arginine is essential for binding and orientates the peptide into a positively charged pocket on c-Cbl. Surprisingly, c-Met bound to Cbl in the reverse direction, which is unprecedented for SH2 domain binding. The necessity of this intrapeptidyl H-bond was confirmed with isothermal titration calorimetry experiments that also showed Sprouty2 to have the highest binding affinity to c-Cbl; this may enable the selective sequestration of c-Cbl from other target proteins.

About this Structure

3BUM is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for a novel intrapeptidyl H-bond and reverse binding of c-Cbl-TKB domain substrates., Ng C, Jackson RA, Buschdorf JP, Sun Q, Guy GR, Sivaraman J, EMBO J. 2008 Feb 14;. PMID:18273061 Page seeded by OCA on Sun May 4 21:07:17 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bum"
Personal tools