6n3u
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n3u OCA], [http://pdbe.org/6n3u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n3u RCSB], [http://www.ebi.ac.uk/pdbsum/6n3u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n3u ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n3u OCA], [http://pdbe.org/6n3u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n3u RCSB], [http://www.ebi.ac.uk/pdbsum/6n3u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n3u ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | HIV-1 protease (PR) cleavage of the Gag polyprotein triggers the assembly of mature, infectious particles. Final cleavage of Gag occurs at the junction helix between the capsid protein CA and the SP1 spacer peptide. Here we used MicroED to delineate the binding interactions of the maturation inhibitor bevirimat (BVM) using very thin frozen-hydrated, 3D microcrystals of a CTD-SP1 Gag construct with and without bound BVM. The 2.9-A MicroED structure revealed that a single BVM molecule stabilizes the six-helix bundle via both electrostatic interactions with the dimethylsuccinyl moiety and hydrophobic interactions with the pentacyclic triterpenoid ring. These results provide insight into the mechanism of action of BVM and related maturation inhibitors that will inform further drug discovery efforts. This study also demonstrates the capabilities of MicroED for structure-based drug design. | ||
| + | |||
| + | MicroED structures of HIV-1 Gag CTD-SP1 reveal binding interactions with the maturation inhibitor bevirimat.,Purdy MD, Shi D, Chrustowicz J, Hattne J, Gonen T, Yeager M Proc Natl Acad Sci U S A. 2018 Dec 10. pii: 1806806115. doi:, 10.1073/pnas.1806806115. PMID:30530702<ref>PMID:30530702</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6n3u" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 08:47, 26 December 2018
MicroED Structure of the CTD-SP1 fragment of HIV-1 Gag with bound maturation inhibitor Bevirimat.
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