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3j4g

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Revision as of 11:59, 23 September 2020

Structure of lysozyme solved by MicroED to 2.9 A

Structural highlights

3j4g is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Activity:	Lysozyme, with EC number 3.2.1.17
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.^[1]

Publication Abstract from PubMed

We demonstrate that it is feasible to determine high-resolution protein structures by electron crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM). Lysozyme microcrystals were frozen on an electron microscopy grid, and electron diffraction data collected to 1.7 A resolution. We developed a data collection protocol to collect a full-tilt series in electron diffraction to atomic resolution. A single tilt series contains up to 90 individual diffraction patterns collected from a single crystal with tilt angle increment of 0.1-1 degrees and a total accumulated electron dose less than 10 electrons per angstrom squared. We indexed the data from three crystals and used them for structure determination of lysozyme by molecular replacement followed by crystallographic refinement to 2.9 A resolution. This proof of principle paves the way for the implementation of a new technique, which we name 'MicroED', that may have wide applicability in structural biology. DOI: http://dx.doi.org/10.7554/eLife.01345.001.

Three-dimensional electron crystallography of protein microcrystals.,Shi D, Nannenga BL, Iadanza MG, Gonen T Elife. 2013 Jan 1;2(0). pii: e01345. doi: 10.7554/eLife.01345. PMID:24252878^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
↑ Shi D, Nannenga BL, Iadanza MG, Gonen T. Three-dimensional electron crystallography of protein microcrystals. Elife. 2013 Jan 1;2(0). pii: e01345. doi: 10.7554/eLife.01345. PMID:24252878 doi:http://dx.doi.org/10.7554/eLife.01345

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j4g"

@@ Line 1: / Line 1: @@
 ==Structure of lysozyme solved by MicroED to 2.9 A==
-<StructureSection load='3j4g' size='340' side='right' caption='[[3j4g]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+<StructureSection load='3j4g' size='340' side='right'caption='[[3j4g]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3j4g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J4G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3j4g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J4G FirstGlance]. <br>
 </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4g OCA], [http://pdbe.org/3j4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j4g RCSB], [http://www.ebi.ac.uk/pdbsum/3j4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j4g ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4g OCA], [http://pdbe.org/3j4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j4g RCSB], [http://www.ebi.ac.uk/pdbsum/3j4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j4g ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 26: / Line 26: @@
 </StructureSection>
 [[Category: Gallus gallus]]
+[[Category: Large Structures]]
 [[Category: Lysozyme]]
 [[Category: Gonen, T]]

3j4g

From Proteopedia

Revision as of 11:59, 23 September 2020

Structure of lysozyme solved by MicroED to 2.9 A

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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