3igc

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==Smallpox virus topoisomerase-DNA transition state==
==Smallpox virus topoisomerase-DNA transition state==
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<StructureSection load='3igc' size='340' side='right' caption='[[3igc]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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<StructureSection load='3igc' size='340' side='right'caption='[[3igc]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3igc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Varv Varv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IGC FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3igc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Varv Varv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IGC FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">H6R, I6R, TOP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10255 VARV])</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">H6R, I6R, TOP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10255 VARV])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3igc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3igc OCA], [http://pdbe.org/3igc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3igc RCSB], [http://www.ebi.ac.uk/pdbsum/3igc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3igc ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3igc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3igc OCA], [https://pdbe.org/3igc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3igc RCSB], [https://www.ebi.ac.uk/pdbsum/3igc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3igc ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TOP1_VAR67 TOP1_VAR67]] Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
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[[https://www.uniprot.org/uniprot/TOP1_VAR67 TOP1_VAR67]] Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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==See Also==
==See Also==
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*[[Smallpox (Variola Virus) - Topoisomerase 1B|Smallpox (Variola Virus) - Topoisomerase 1B]]
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*[[Topoisomerase 3D structures|Topoisomerase 3D structures]]
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*[[Topoisomerase|Topoisomerase]]
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*[[Variola Topoisomerase 1B|Variola Topoisomerase 1B]]
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: DNA topoisomerase]]
[[Category: DNA topoisomerase]]
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[[Category: Large Structures]]
[[Category: Varv]]
[[Category: Varv]]
[[Category: Bushman, F D]]
[[Category: Bushman, F D]]

Revision as of 12:10, 13 October 2021

Smallpox virus topoisomerase-DNA transition state

PDB ID 3igc

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