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| | ==Crystal structure of procruciferin, 11S globulin from Brassica napus== | | ==Crystal structure of procruciferin, 11S globulin from Brassica napus== |
| - | <StructureSection load='3kgl' size='340' side='right' caption='[[3kgl]], [[Resolution|resolution]] 2.98Å' scene=''> | + | <StructureSection load='3kgl' size='340' side='right'caption='[[3kgl]], [[Resolution|resolution]] 2.98Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3kgl]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Brana Brana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KGL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KGL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3kgl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_napus Brassica napus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KGL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.981Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Plant Gene ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3708 BRANA])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kgl OCA], [http://pdbe.org/3kgl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kgl RCSB], [http://www.ebi.ac.uk/pdbsum/3kgl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kgl ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kgl OCA], [https://pdbe.org/3kgl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kgl RCSB], [https://www.ebi.ac.uk/pdbsum/3kgl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kgl ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7XB53_BRANA Q7XB53_BRANA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Brana]] | |
| - | [[Category: Maruyama, N]] | |
| - | [[Category: Mikami, B]] | |
| - | [[Category: Tandang-Silvas, M R]] | |
| - | [[Category: Utsumi, S]] | |
| - | [[Category: 11s seed globulin]] | |
| | [[Category: Brassica napus]] | | [[Category: Brassica napus]] |
| - | [[Category: Plant protein]] | + | [[Category: Large Structures]] |
| - | [[Category: Procruciferin]] | + | [[Category: Maruyama N]] |
| - | [[Category: Rapeseed]] | + | [[Category: Mikami B]] |
| - | [[Category: Seed storage protein]] | + | [[Category: Tandang-Silvas MR]] |
| - | [[Category: Storage protein]] | + | [[Category: Utsumi S]] |
| Structural highlights
Function
Q7XB53_BRANA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The crystal structures of two pro-11S globulins namely: rapeseed procruciferin and pea prolegumin are presented here. We have extensively compared them with the other known structures of plant seed 11S and 7S globulins. In general, the disordered regions in the crystal structures among the 11S globulins correspond to their five variable regions. Variable region III of procruciferin is relatively short and is in a loop conformation. This region is highly disordered in other pro-11S globulin crystals. Local helical and strand variations also occur across the group despite general structure conservation. We showed how these variations may alter specific physicochemical, functional and physiological properties. Aliphatic hydrophobic residues on the molecular surface correlate well with Tm values of the globulins. We also considered other structural features that were reported to influence thermal stability but no definite conclusion was drawn since each factor has additive or subtractive effect. Comparison between proA3B4 and mature A3B4 revealed an increase in r.m.s.d. values near variable regions II and IV. Both regions are on the IE face. Secondary structure based alignment of 11S and 7S globulins revealed 16 identical residues. Based on proA3B4 sequence, Pro60, Gly128, Phe163, Phe208, Leu213, Leu227, Ile237, Pro382, Val404, Pro425 and Val 466 are involved in trimer formation and stabilization. Gly28, Gly74, Asp135, Gly349 and Gly397 are involved in correct globular folding.
Conservation and divergence on plant seed 11S globulins based on crystal structures.,Tandang-Silvas MR, Fukuda T, Fukuda C, Prak K, Cabanos C, Kimura A, Itoh T, Mikami B, Utsumi S, Maruyama N Biochim Biophys Acta. 2010 Jul;1804(7):1432-42. Epub 2010 Mar 6. PMID:20215054[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tandang-Silvas MR, Fukuda T, Fukuda C, Prak K, Cabanos C, Kimura A, Itoh T, Mikami B, Utsumi S, Maruyama N. Conservation and divergence on plant seed 11S globulins based on crystal structures. Biochim Biophys Acta. 2010 Jul;1804(7):1432-42. Epub 2010 Mar 6. PMID:20215054 doi:10.1016/j.bbapap.2010.02.016
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