3htc

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[[Image:3htc.jpg|left|200px]]
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{{Structure
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3htc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3htc OCA], [http://www.ebi.ac.uk/pdbsum/3htc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3htc RCSB]</span>
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'''THE STRUCTURE OF A COMPLEX OF RECOMBINANT HIRUDIN AND HUMAN ALPHA-THROMBIN'''
'''THE STRUCTURE OF A COMPLEX OF RECOMBINANT HIRUDIN AND HUMAN ALPHA-THROMBIN'''
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[[Category: Rydel, T J.]]
[[Category: Rydel, T J.]]
[[Category: Tulinsky, A.]]
[[Category: Tulinsky, A.]]
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[[Category: hydrolase(serine protease)]]
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Revision as of 19:04, 4 May 2008

Image:3htc.jpg

Template:STRUCTURE 3htc

THE STRUCTURE OF A COMPLEX OF RECOMBINANT HIRUDIN AND HUMAN ALPHA-THROMBIN


Overview

The crystallographic structure of a recombinant hirudin-thrombin complex has been solved at 2.3 angstrom (A) resolution. Hirudin consists of an NH2-terminal globular domain and a long (39 A) COOH-terminal extended domain. Residues Ile1 to Tyr3 of hirudin form a parallel beta-strand with Ser214 to Glu217 of thrombin with the nitrogen atom of Ile1 making a hydrogen bond with Ser195 O gamma atom of the catalytic site, but the specificity pocket of thrombin is not involved in the interaction. The COOH-terminal segment makes numerous electrostatic interactions with an anion-binding exosite of thrombin, whereas the last five residues are in a helical loop that forms many hydrophobic contacts. In all, 27 of the 65 residues of hirudin have contacts less than 4.0 A with thrombin (10 ion pairs and 23 hydrogen bonds). Such abundant interactions may account for the high affinity and specificity of hirudin.

About this Structure

3HTC is a Protein complex structure of sequences from Hirudinaria manillensis and Homo sapiens. Full crystallographic information is available from OCA.

Reference

The structure of a complex of recombinant hirudin and human alpha-thrombin., Rydel TJ, Ravichandran KG, Tulinsky A, Bode W, Huber R, Roitsch C, Fenton JW 2nd, Science. 1990 Jul 20;249(4966):277-80. PMID:2374926 Page seeded by OCA on Sun May 4 22:04:15 2008

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