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| | ==Crystal structure of the SRP19/S-domain SRP RNA complex of Sulfolobus solfataricus== | | ==Crystal structure of the SRP19/S-domain SRP RNA complex of Sulfolobus solfataricus== |
| - | <StructureSection load='3ktw' size='340' side='right' caption='[[3ktw]], [[Resolution|resolution]] 3.20Å' scene=''> | + | <StructureSection load='3ktw' size='340' side='right'caption='[[3ktw]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ktw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KTW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ktw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KTW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ktv|3ktv]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">srp19, SSO0165 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ktw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktw OCA], [https://pdbe.org/3ktw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ktw RCSB], [https://www.ebi.ac.uk/pdbsum/3ktw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ktw ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ktw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktw OCA], [http://pdbe.org/3ktw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ktw RCSB], [http://www.ebi.ac.uk/pdbsum/3ktw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ktw ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SRP19_SULSO SRP19_SULSO]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP (By similarity). | + | [https://www.uniprot.org/uniprot/SRP19_SACS2 SRP19_SACS2] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.[HAMAP-Rule:MF_00305] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 35091]] | + | [[Category: Large Structures]] |
| - | [[Category: Bange, G]] | + | [[Category: Saccharolobus solfataricus]] |
| - | [[Category: Bozkurt, G]] | + | [[Category: Bange G]] |
| - | [[Category: Sinning, I]] | + | [[Category: Bozkurt G]] |
| - | [[Category: Wild, K]] | + | [[Category: Sinning I]] |
| - | [[Category: 7s rna]]
| + | [[Category: Wild K]] |
| - | [[Category: Asymmetric loop]]
| + | |
| - | [[Category: Ribonucleoprotein complex]]
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| - | [[Category: Rna-binding]]
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| - | [[Category: Rna-rna binding protein complex]]
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| - | [[Category: Rna-rna tertiary interaction]]
| + | |
| - | [[Category: Signal recognition particle]]
| + | |
| - | [[Category: Structural rna]]
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| Structural highlights
Function
SRP19_SACS2 Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.[HAMAP-Rule:MF_00305]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The signal recognition particle (SRP) is a conserved ribonucleoprotein (RNP) complex that co-translationally targets membrane and secretory proteins to membranes. The assembly of the particle depends on the proper folding of the SRP RNA, which in mammalia and archaea involves an induced-fit mechanism within helices 6 and 8 in the S domain of SRP. The two helices are juxtaposed and clamped together upon binding of the SRP19 protein to their apices. In the current assembly paradigm, archaeal SRP19 causes the asymmetric loop of helix 8 to bulge out and expose the binding platform for the key player SRP54. Based on a heterologous archaeal SRP19-human SRP RNA structure, mammalian SRP19 was thought not to be able to induce this change, thus explaining the different requirements of SRP19 for SRP54 recruitment. In contrast, the crystal structures of a crenarchaeal and the all-human SRP19-SRP RNA binary complexes presented here show that the asymmetric loop is bulged out in both binary complexes. Differences in SRP assembly between mammalia and archaea are therefore independent of SRP19 and are based on differences in SRP RNA itself. A new SRP-assembly scheme is presented.
Structural insights into the assembly of the human and archaeal signal recognition particles.,Wild K, Bange G, Bozkurt G, Segnitz B, Hendricks A, Sinning I Acta Crystallogr D Biol Crystallogr. 2010 Mar;66(Pt 3):295-303. Epub 2010, Feb 12. PMID:20179341[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wild K, Bange G, Bozkurt G, Segnitz B, Hendricks A, Sinning I. Structural insights into the assembly of the human and archaeal signal recognition particles. Acta Crystallogr D Biol Crystallogr. 2010 Mar;66(Pt 3):295-303. Epub 2010, Feb 12. PMID:20179341 doi:10.1107/S0907444910000879
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