3l40

<StructureSection load='3l40' size='340' side='right' caption='[[3l40]], [[Resolution|resolution]] 1.55&Aring;' scene=''>

<table><tr><td colspan='2'>[[3l40]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L40 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L40 FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1jnx|1jnx]], [[3hue|3hue]], [[2r1z|2r1z]], [[1t2v|1t2v]], [[2azm|2azm]], [[3l41|3l41]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">brc1, SPBC582.05c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l40 OCA], [http://pdbe.org/3l40 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l40 RCSB], [http://www.ebi.ac.uk/pdbsum/3l40 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l40 ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/BRC1_SCHPO BRC1_SCHPO]] Required for mitotic fidelity, specifically in the G2 phase of the cell cycle. Plays a role in chromatin organization.

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== Publication Abstract from PubMed ==

ATM(Tel1) and ATR(Rad3) checkpoint kinases phosphorylate the C-terminus of histone H2AX (H2A in yeasts) in chromatin flanking DNA damage, establishing a recruitment platform for checkpoint and repair proteins. Phospho-H2A/X (gammaH2A/X)-binding proteins at double-strand breaks (DSBs) have been characterized, but those required for replication stress responses are unknown. Here, we present genetic, biochemical, small angle X-ray scattering (SAXS), and X-ray structural studies of the Schizosaccharomyces pombe Brc1, a 6-BRCT-domain protein that is structurally related to Saccharomyces cerevisiae Rtt107 and mammalian PTIP. Brc1 binds gammaH2A to form spontaneous and DNA damage-induced nuclear foci. Spontaneous Brc1 foci colocalize with ribosomal DNA repeats, a region prone to fork pausing and genomic instability, whereas DNA damage-induced Brc1 foci colocalize with DSB response factors. gammaH2A binding is critical for Brc1 function. The 1.45 A resolution crystal structure of Brc1-gammaH2A complex shows how variable BRCT insertion loops sculpt tandem-BRCT phosphoprotein-binding pockets to facilitate unique phosphoprotein-interaction specificities, and unveils an acidic DNA-mimicking Brc1 surface. From these results, Brc1 docking to gammaH2A emerges as a critical chromatin-specific response to replication-associated DNA damage.

gammaH2A binds Brc1 to maintain genome integrity during S-phase.,Williams JS, Williams RS, Dovey CL, Guenther G, Tainer JA, Russell P EMBO J. 2010 Mar 17;29(6):1136-48. Epub 2010 Jan 21. PMID:20094029<ref>PMID:20094029</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3l40" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Cbs 356]]

[[Category: Guenther, G]]

[[Category: Tainer, J A]]

[[Category: Williams, J S]]

[[Category: Williams, R S]]

[[Category: Brc1]]

[[Category: Tandem brct repeat]]