2jes

From Proteopedia

(Difference between revisions)

Revision as of 06:56, 7 October 2020

Portal protein (gp6) from bacteriophage SPP1

Structural highlights

2jes is a 26 chain structure with sequence from Bpspp. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PORTL_BPSPP] Forms the portal vertex of the capsid (PubMed:17363899). This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel. Binds to the terminase subunits to form the packaging machine.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Tailed bacteriophages and herpesviruses load their capsids with DNA through a tunnel formed by the portal protein assembly. Here we describe the X-ray structure of the bacteriophage SPP1 portal protein in its isolated 13-subunit form and the pseudoatomic structure of a 12-subunit assembly. The first defines the DNA-interacting segments (tunnel loops) that pack tightly against each other forming the most constricted part of the tunnel; the second shows that the functional dodecameric state must induce variability in the loop positions. Structural observations together with geometrical constraints dictate that in the portal-DNA complex, the loops form an undulating belt that fits and tightly embraces the helical DNA, suggesting that DNA translocation is accompanied by a 'mexican wave' of positional and conformational changes propagating sequentially along this belt.

Structural framework for DNA translocation via the viral portal protein.,Lebedev AA, Krause MH, Isidro AL, Vagin AA, Orlova EV, Turner J, Dodson EJ, Tavares P, Antson AA EMBO J. 2007 Apr 4;26(7):1984-94. Epub 2007 Mar 15. PMID:17363899^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lebedev AA, Krause MH, Isidro AL, Vagin AA, Orlova EV, Turner J, Dodson EJ, Tavares P, Antson AA. Structural framework for DNA translocation via the viral portal protein. EMBO J. 2007 Apr 4;26(7):1984-94. Epub 2007 Mar 15. PMID:17363899
↑ Lebedev AA, Krause MH, Isidro AL, Vagin AA, Orlova EV, Turner J, Dodson EJ, Tavares P, Antson AA. Structural framework for DNA translocation via the viral portal protein. EMBO J. 2007 Apr 4;26(7):1984-94. Epub 2007 Mar 15. PMID:17363899

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jes"

@@ Line 1: / Line 1: @@
 ==Portal protein (gp6) from bacteriophage SPP1==
-<StructureSection load='2jes' size='340' side='right' caption='[[2jes]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
+<StructureSection load='2jes' size='340' side='right'caption='[[2jes]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jes]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpspp Bpspp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JES FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jes]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpspp Bpspp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JES OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2JES FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jes OCA], [http://pdbe.org/2jes PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jes RCSB], [http://www.ebi.ac.uk/pdbsum/2jes PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jes ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2jes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jes OCA], [http://pdbe.org/2jes PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jes RCSB], [http://www.ebi.ac.uk/pdbsum/2jes PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jes ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 37: / Line 37: @@
 </StructureSection>
 [[Category: Bpspp]]
+[[Category: Large Structures]]
 [[Category: Antson, A A]]
 [[Category: Dodson, E J]]

2jes

From Proteopedia

Revision as of 06:56, 7 October 2020

Portal protein (gp6) from bacteriophage SPP1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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