472d
From Proteopedia
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[[Image:472d.gif|left|200px]] | [[Image:472d.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_472d| PDB=472d | SCENE= }} | |
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'''STRUCTURE OF AN OCTAMER RNA WITH TANDEM GG/UU MISPAIRS''' | '''STRUCTURE OF AN OCTAMER RNA WITH TANDEM GG/UU MISPAIRS''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=472D OCA]. | |
==Reference== | ==Reference== | ||
Synthesis and crystal structure of an octamer RNA r(guguuuac)/r(guaggcac) with G.G/U.U tandem wobble base pairs: comparison with other tandem G.U pairs., Deng J, Sundaralingam M, Nucleic Acids Res. 2000 Nov 1;28(21):4376-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11058138 11058138] | Synthesis and crystal structure of an octamer RNA r(guguuuac)/r(guaggcac) with G.G/U.U tandem wobble base pairs: comparison with other tandem G.U pairs., Deng J, Sundaralingam M, Nucleic Acids Res. 2000 Nov 1;28(21):4376-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11058138 11058138] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Deng, J.]] | [[Category: Deng, J.]] | ||
[[Category: Sundaralingam, M.]] | [[Category: Sundaralingam, M.]] | ||
| - | [[Category: | + | [[Category: Gg/uu mispair]] |
| - | [[Category: | + | [[Category: Ribonucleic acid]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:19:32 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:19, 4 May 2008
STRUCTURE OF AN OCTAMER RNA WITH TANDEM GG/UU MISPAIRS
Overview
We have determined the crystal structure of the RNA octamer duplex r(guguuuac)/r(guaggcac) with a tandem wobble pair, G.G/U.U (motif III), to compare it with U.G/G.U (motif I) and G.U/U.G (motif II) and to better understand their relative stabilities. The crystal belongs to the rhombohedral space group R3. The hexagonal unit cell dimensions are a = b = 41.92 A, c = 56.41 A, and gamma = 120 degrees, with one duplex in the asymmetric unit. The structure was solved by the molecular replacement method at 1.9 A resolution and refined to a final R: factor of 19.9% and R(free) of 23.3% for 2862 reflections in the resolution range 10.0-1.9 A with F >/= 2sigma(F). The final model contains 335 atoms for the RNA duplex and 30 water molecules. The A-RNA stacks in the familiar head-to-tail fashion forming a pseudo-continuous helix. The uridine bases of the tandem U.G pairs have slipped towards the minor groove relative to the guanine bases and the uridine O2 atoms form bifurcated hydrogen bonds with the N1 and N2 of guanines. The N2 of guanine and O2 of uridine do not bridge the 'locked' water molecule in the minor groove, as in motifs I and II, but are bridged by water molecules in the major groove. A comparison of base stacking stabilities of motif III with motifs I and II confirms the result of thermodynamic studies, motif I > motif III > motif II.
About this Structure
Full crystallographic information is available from OCA.
Reference
Synthesis and crystal structure of an octamer RNA r(guguuuac)/r(guaggcac) with G.G/U.U tandem wobble base pairs: comparison with other tandem G.U pairs., Deng J, Sundaralingam M, Nucleic Acids Res. 2000 Nov 1;28(21):4376-81. PMID:11058138 Page seeded by OCA on Sun May 4 22:19:32 2008
