3lrv

<StructureSection load='3lrv' size='340' side='right' caption='[[3lrv]], [[Resolution|resolution]] 2.60&Aring;' scene=''>

<table><tr><td colspan='2'>[[3lrv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LRV FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRP19, PSO4, YLL036C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lrv OCA], [http://pdbe.org/3lrv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lrv RCSB], [http://www.ebi.ac.uk/pdbsum/3lrv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lrv ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/PRP19_YEAST PRP19_YEAST]] Involved in pre-mRNA splicing. Acts a central component of the NTC complex (or PRP19-associated complex) that associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. Involved in DNA repair.

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== Publication Abstract from PubMed ==

Prp19 is a member of the WD40 repeat family of E3 ubiquitin ligases and a conserved eukaryotic RNA splicing factor essential for activation and stabilization of the spliceosome. To understand the role of the WD40 repeat domain of Prp19 we have determined its structure using X-ray crystallography. The domain has a distorted seven bladed WD40 architecture with significant asymmetry due to irregular packing of blades one and seven into the core of the WD40 domain. Structure-based mutagenesis identified a highly conserved surface centered around blade five that is required for the physical interaction between Prp19 and Cwc2, another essential splicing factor. This region is found to be required for Prp19 function and yeast viability. Experiments in vitro and in vivo demonstrate that two molecules of Cwc2 bind to the Prp19 tetramer. These coupled structural and functional studies provide a model for the functional architecture of Prp19.

The Prp19 WD40 domain contains a conserved protein interaction region essential for its function.,Vander Kooi CW, Ren L, Xu P, Ohi MD, Gould KL, Chazin WJ Structure. 2010 May 12;18(5):584-93. PMID:20462492<ref>PMID:20462492</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3lrv" style="background-color:#fffaf0;"></div>

*[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]]

*[[Prp19|Prp19]]

[[Category: Atcc 18824]]

[[Category: Chazin, W J]]

[[Category: Kooi, C W.Vander]]

[[Category: Dna damage]]

[[Category: Wd40]]