User:Estelle Blochouse/ Sandbox 1497

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== Function ==
== Function ==
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Multicopper oxidases are enzymes involved in copper homeostasis. Copper as many metal ions is used in multiple biological processes such as detoxification of oxygen free radicals and pigmentation. However copper present in a cell bot bounded to a protein if harmfull and can cause cellular damage. It need to be regulated.
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Multicopper oxidases are enzymes involved in copper homeostasis. Copper as many metal ions is used in multiple biological processes such as detoxification of oxygen free radicals and pigmentation. However copper present in a cell bot bounded to a protein if harmfull and can cause cellular damage. It need to be regulated.
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Multicopper oxidase might also be involved in the regulation of metal transport.
Multicopper oxidase might also be involved in the regulation of metal transport.
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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Multicopper Oxidase CueO [[4e9s]] is a protein containing one chain with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E9S FirstGlance]. <br>
<table><tr><td colspan='2'>Multicopper Oxidase CueO [[4e9s]] is a protein containing one chain with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E9S FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>Acetate ion</scene>[[Image:Act.jpg|thumb|middle|'''Figure 1:''' Acetate ion.<ref>PDB image</ref>]], <scene name='pdbligand=CU:COPPER+(II)+ION'>Copper</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>Acetate ion</scene>[[Image:Act.jpg|thumb|middle|'''Figure 1:''' Acetate ion.<ref>RCSB PDB</ref>]], <scene name='pdbligand=CU:COPPER+(II)+ION'>Copper</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4e9p|4e9p]], [[4e9q|4e9q]], [[4e9r|4e9r]], [[4e9t|4e9t]]</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4e9p|4e9p]], [[4e9q|4e9q]], [[4e9r|4e9r]], [[4e9t|4e9t]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cueO, yacK, b0123, JW0119 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cueO, yacK, b0123, JW0119 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
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</table>
</table>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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In the chain, 5 ligands are present: an acetate ion and 4 copper ions.
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-[[Image:Cu1001.jpg|'''Figure 2:''' Copper 1001<ref>RCSB PDB</ref>]]: The copper ion is bound thanks to 3 metal protein interactions with H443, H505 and C500, structure stabilised by L502, M510 by hydrogen bonds
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-[[Image:Cu1002.jpg|'''Figure 3:''' Copper 1002<ref>RCSB PDB</ref>]]: 2 atoms of Cu, bonds twice by metal interaction (2x3) with 3 histidine : H501, H103, H141 stabilised by hydrophobic contact with W139
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-[[Image:Cu1003.jpg|'''Figure 4:''' Copper 1003<ref>RCSB PDB</ref>]]: 2 CU bond with 3histidine : H499, H143, H448 by 2 metal interactions. h448 is stabilised by Pi interactions with [CU]1004 and H101
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-[[Image:Cu1004.jpg|'''Figure 5:''' Copper 1004<ref>RCSB PDB</ref>]]: one single atome of CU bonds once by metal interactions with 2 H ( and one ACT[1005]) : H101 and H446 stabilised by Pi interactions with H103 and H448. [CU]1004 has Pi interactions with H103 and H448. H113 and H446 Pi interactions
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-[[Image:Act1005.jpg|'''Figure 6:''' Acetate ion 1005<ref>RCSB PDB</ref>]]: is linked by hydrogen bonds to G104 and G449
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[CU]1003, [CU]1002, [CU]1004 and ACT[1005] are near in the space, the 3 CU form a triangle.
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This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

Revision as of 21:38, 10 January 2019

Multicopper Oxidase CueO (4e9s)

4e9s, resolution 1.06Å

Drag the structure with the mouse to rotate

References

  1. ↑ EMBL-EBI, Family: Cu-oxidase (PF00394), Summary: Multicopper oxidase, http://pfam.xfam.org/family/Cu-oxidase
  2. ↑ <ref>PMID:9556453</ref>
  3. ↑ <ref>PMID:9556453</ref>
  4. ↑ <ref>PMID:9556453</ref>
  5. ↑ RCSB PDB
  6. ↑ RCSB PDB
  7. ↑ RCSB PDB
  8. ↑ RCSB PDB
  9. ↑ RCSB PDB
  10. ↑ RCSB PDB

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Estelle Blochouse

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