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6e5o
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==CasX ternary complex with 30bp target DNA== | |
| + | <StructureSection load='6e5o' size='340' side='right' caption='[[6e5o]], [[Resolution|resolution]] 3.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6e5o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Deltaproteobacteria_bacterium Deltaproteobacteria bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E5O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E5O FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DD725_10130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2026735 Deltaproteobacteria bacterium])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e5o OCA], [http://pdbe.org/6e5o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e5o RCSB], [http://www.ebi.ac.uk/pdbsum/6e5o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e5o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against invading nucleic acids, and function as powerful tools for genome editing in a wide range of organisms. Here we reveal the underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR-CasX, which uses unique structures for programmable double-stranded DNA binding and cleavage. Biochemical and in vivo data demonstrate that CasX is active for Escherichia coli and human genome modification. Eight cryo-electron microscopy structures of CasX in different states of assembly with its guide RNA and double-stranded DNA substrates reveal an extensive RNA scaffold and a domain required for DNA unwinding. These data demonstrate how CasX activity arose through convergent evolution to establish an enzyme family that is functionally separate from both Cas9 and Cas12a. | ||
| - | + | CasX enzymes comprise a distinct family of RNA-guided genome editors.,Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA Nature. 2019 Feb;566(7743):218-223. doi: 10.1038/s41586-019-0908-x. Epub 2019 Feb, 4. PMID:30718774<ref>PMID:30718774</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6e5o" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Deltaproteobacteria bacterium]] | ||
| + | [[Category: Doudna, J A]] | ||
| + | [[Category: Liu, J J]] | ||
| + | [[Category: Nogales, E]] | ||
| + | [[Category: Orlova, N]] | ||
| + | [[Category: Casx]] | ||
| + | [[Category: Crispr]] | ||
| + | [[Category: Rna binding protein]] | ||
| + | [[Category: Rna binding protein-rna-dna complex]] | ||
| + | [[Category: Sgrna]] | ||
| + | [[Category: Target dna]] | ||
Revision as of 08:37, 21 February 2019
CasX ternary complex with 30bp target DNA
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