6iyn
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution structure of camelid nanobody Nb26 against aflatoxin B1== | |
| + | <StructureSection load='6iyn' size='340' side='right'caption='[[6iyn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6iyn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Alpaca Alpaca]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IYN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IYN FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iyn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iyn OCA], [http://pdbe.org/6iyn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iyn RCSB], [http://www.ebi.ac.uk/pdbsum/6iyn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iyn ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nanobodies (Nbs) are the variable domain of the heavy-chain antibodies produced from Camelidae, which possess comparable binding affinities and specificity to conventional antibodies. Nbs have become valuable and versatile tools for numerous biotechnology applications due to their small size (12-15 kDa), high solubility, exceptional stability, and facile genetic manipulation. The interactions between Nbs and protein antigens have been well-studied, but less work has been done to characterize their ability to bind small molecule haptens. Here we present the backbone and side-chain assignments of the (1)H, (13)C and (15)N resonances of Nb26 (123 amino acids), a nanobody that recognizes the hapten aflatoxin B1 (AFB1). These assignments are preliminary work towards the determination of the structure of free Nb26 using NMR spectroscopy, which will provide useful information about the complex structure of "Nb26-AFB1" and the recognition mechanism about how Nb26 binds to AFB1. | ||
| - | + | Chemical shift assignments of a camelid nanobody against aflatoxin B1.,Nie Y, Li S, Zhu J, Hu R, Liu M, He T, Yang Y Biomol NMR Assign. 2019 Apr;13(1):75-78. doi: 10.1007/s12104-018-9855-y. Epub, 2018 Oct 16. PMID:30328057<ref>PMID:30328057</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6iyn" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Alpaca]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: He, T]] | ||
[[Category: Hu, R]] | [[Category: Hu, R]] | ||
| - | [[Category: Li, S | + | [[Category: Li, S L]] |
[[Category: Nie, Y]] | [[Category: Nie, Y]] | ||
| - | [[Category: Yang, Y | + | [[Category: Yang, Y H]] |
[[Category: Zhu, J]] | [[Category: Zhu, J]] | ||
| - | [[Category: | + | [[Category: Aflatoxin b1]] |
| + | [[Category: Aflatoxin b1-binding protein]] | ||
| + | [[Category: Camelid antibody]] | ||
| + | [[Category: Nanobody]] | ||
Revision as of 07:52, 10 April 2019
Solution structure of camelid nanobody Nb26 against aflatoxin B1
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Categories: Alpaca | Large Structures | He, T | Hu, R | Li, S L | Nie, Y | Yang, Y H | Zhu, J | Aflatoxin b1 | Aflatoxin b1-binding protein | Camelid antibody | Nanobody
