6j4h
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of maltotriose-complex of PulA-G680L mutant from Klebsiella pneumoniae== | |
| + | <StructureSection load='6j4h' size='340' side='right'caption='[[6j4h]], [[Resolution|resolution]] 1.64Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6j4h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J4H FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.641Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRD_900009:alpha-maltotriose'>PRD_900009</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j4h OCA], [https://pdbe.org/6j4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j4h RCSB], [https://www.ebi.ac.uk/pdbsum/6j4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j4h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/W9BQ28_KLEPN W9BQ28_KLEPN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Klebsiella pneumoniae pullulanase (KPP) belongs to glycoside hydrolase family 13 subfamily 13 (GH13_13) and is the only enzyme that is reported to perform an induced-fit motion of the active-site loop (residues 706-710). Comparison of pullulanase structures indicated that only KPP has Leu680 present behind the loop, in contrast to the glycine found in other GH13_13 members. Analysis of the structure and activity of recombinant pullulanase from K. pneumoniae ATCC 9621 (rKPP) and its mutant (rKPP-G680L) indicated that the side chain of residue 680 is important for the induced-fit motion of the loop 706-710 and alters the binding affinity of the substrate. | ||
| - | + | Relationship between the induced-fit loop and the activity of Klebsiella pneumoniae pullulanase.,Saka N, Malle D, Iwamoto H, Takahashi N, Mizutani K, Mikami B Acta Crystallogr D Struct Biol. 2019 Sep 1;75(Pt 9):792-803. doi: , 10.1107/S2059798319010660. Epub 2019 Aug 22. PMID:31478902<ref>PMID:31478902</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6j4h" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Takahashi | + | </StructureSection> |
| + | [[Category: Klebsiella pneumoniae]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Iwamoto H]] | ||
| + | [[Category: Mikami B]] | ||
| + | [[Category: Mizutani K]] | ||
| + | [[Category: Saka N]] | ||
| + | [[Category: Takahashi N]] | ||
Current revision
Crystal Structure of maltotriose-complex of PulA-G680L mutant from Klebsiella pneumoniae
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