6n4v
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==CryoEM structure of Leviviridae PP7 WT coat protein dimer capsid (PP7PP7-WT)== | |
| + | <StructureSection load='6n4v' size='340' side='right'caption='[[6n4v]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6n4v]] is a 120 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N4V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N4V FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n4v OCA], [http://pdbe.org/6n4v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n4v RCSB], [http://www.ebi.ac.uk/pdbsum/6n4v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n4v ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | As self-assembling polyvalent nanoscale structures that can tolerate substantial genetic and chemical modification, virus-like particles are useful in a variety of fields. Here we describe the genetic modification and structural characterization of the Leviviridae PP7 capsid protein as a platform for the presentation of functional polypeptides. This particle was shown to tolerate the display of sequences from 1 kDa (a cell penetrating peptide) to 14 kDa (the Fc-binding double Z-domain) on its exterior surface as C-terminal genetic fusions to the coat protein. In addition, a dimeric construct allowed the presentation of exogenous loops between capsid monomers and the simultaneous presentation of two different peptides at different positions on the icosahedral structure. The PP7 particle is thereby significantly more tolerant of these types of polypeptide additions than Qbeta and MS2, the other Leviviridae-derived VLPs in common use. | ||
| - | + | Engineering the PP7 Virus Capsid as a Peptide Display Platform.,Zhao L, Kopylov M, Potter CS, Carragher B, Finn MG ACS Nano. 2019 Mar 26. doi: 10.1021/acsnano.8b09683. PMID:30912918<ref>PMID:30912918</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 6n4v" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Carragher, B]] | [[Category: Carragher, B]] | ||
| + | [[Category: Finn, M G]] | ||
[[Category: Kopylov, M]] | [[Category: Kopylov, M]] | ||
[[Category: Liangjun, Z]] | [[Category: Liangjun, Z]] | ||
| - | [[Category: | + | [[Category: Potter, C S]] |
| + | [[Category: Biotechnology]] | ||
| + | [[Category: Drug delivery]] | ||
| + | [[Category: Icosahedral]] | ||
| + | [[Category: Pp7]] | ||
| + | [[Category: T4]] | ||
| + | [[Category: Vaccine]] | ||
| + | [[Category: Virus like particle]] | ||
Revision as of 07:08, 3 April 2019
CryoEM structure of Leviviridae PP7 WT coat protein dimer capsid (PP7PP7-WT)
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Categories: Large Structures | Carragher, B | Finn, M G | Kopylov, M | Liangjun, Z | Potter, C S | Biotechnology | Drug delivery | Icosahedral | Pp7 | T4 | Vaccine | Virus like particle
