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6a2h

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Revision as of 06:56, 27 March 2019

Architectural roles of Cren7 in folding crenarchaeal chromatin filament

Structural highlights

6a2h is a 3 chain structure with sequence from Sacs2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	creN7, SSO6901 (SACS2)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CREN7_SULSO] A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.^[1]

Publication Abstract from PubMed

Archaea have evolved various strategies in chromosomal organization. While histone homologues exist in most archaeal phyla, Cren7 is a chromatin protein conserved in the Crenarchaeota. Here, we show that Cren7 preferentially binds DNA with AT-rich sequences over that with GC-rich sequences with a binding size of 6~7 bp. Structural studies of Cren7 in complex with either an 18-bp or a 20-bp double-stranded DNA fragment reveal that Cren7 binds to the minor groove of DNA as monomers in a head-to-tail manner. The neighboring Cren7 monomers are located on the opposite sides of the DNA duplex, with each introducing a single-step sharp kink by intercalation of the hydrophobic side chain of Leu28, bending the DNA into an S-shape conformation. A structural model for the chromatin fiber folded by Cren7 was established and verified by the analysis of cross-linked Cren7-DNA complexes by atomic force microscopy. Our results suggest that Cren7 differs significantly from Sul7, another chromatin protein conserved among Sulfolobus species, in both DNA binding and deformation. These data shed significant light on the strategy of chromosomal DNA organization in crenarchaea.

Architectural roles of Cren7 in folding crenarchaeal chromatin filament.,Zhang Z, Zhao M, Chen Y, Wang L, Liu Q, Dong Y, Gong Y, Huang L Mol Microbiol. 2018 Nov 30. doi: 10.1111/mmi.14173. PMID:30499242^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Guo L, Feng Y, Zhang Z, Yao H, Luo Y, Wang J, Huang L. Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea. Nucleic Acids Res. 2008 Mar;36(4):1129-37. Epub 2007 Dec 20. PMID:18096617 doi:10.1093/nar/gkm1128
↑ Zhang Z, Zhao M, Chen Y, Wang L, Liu Q, Dong Y, Gong Y, Huang L. Architectural roles of Cren7 in folding crenarchaeal chromatin filament. Mol Microbiol. 2018 Nov 30. doi: 10.1111/mmi.14173. PMID:30499242 doi:http://dx.doi.org/10.1111/mmi.14173

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6a2h"

@@ Line 1: / Line 1: @@
 ==Architectural roles of Cren7 in folding crenarchaeal chromatin filament==
-<StructureSection load='6a2h' size='340' side='right' caption='[[6a2h]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='6a2h' size='340' side='right'caption='[[6a2h]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6a2h]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A2H FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6a2h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A2H FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2h OCA], [http://pdbe.org/6a2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a2h RCSB], [http://www.ebi.ac.uk/pdbsum/6a2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2h ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">creN7, SSO6901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2h OCA], [http://pdbe.org/6a2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a2h RCSB], [http://www.ebi.ac.uk/pdbsum/6a2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2h ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
+[[Category: Sacs2]]
 [[Category: Chen, Y Y]]
 [[Category: Dong, Y H]]

6a2h

From Proteopedia

Revision as of 06:56, 27 March 2019

Architectural roles of Cren7 in folding crenarchaeal chromatin filament

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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