6a6n
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of an inward-open apo state of the eukaryotic ABC multidrug transporter CmABCB1== | ==Crystal structure of an inward-open apo state of the eukaryotic ABC multidrug transporter CmABCB1== | ||
| - | <StructureSection load='6a6n' size='340' side='right' caption='[[6a6n]], [[Resolution|resolution]] 3.02Å' scene=''> | + | <StructureSection load='6a6n' size='340' side='right'caption='[[6a6n]], [[Resolution|resolution]] 3.02Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6a6n]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A6N OCA]. For a <b>guided tour on the structure components</b> use [http:// | + | <table><tr><td colspan='2'>[[6a6n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyam1 Cyam1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A6N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6A6N FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CYME_CMD148C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=280699 CYAM1])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6a6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a6n OCA], [http://pdbe.org/6a6n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a6n RCSB], [http://www.ebi.ac.uk/pdbsum/6a6n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a6n ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | P-glycoprotein extrudes a large variety of xenobiotics from the cell, thereby protecting tissues from their toxic effects. The machinery underlying unidirectional multidrug pumping remains unknown, largely due to the lack of high-resolution structural information regarding the alternate conformational states of the molecule. Here we report a pair of structures of homodimeric P-glycoprotein: an outward-facing conformational state with bound nucleotide and an inward-facing apo state, at resolutions of 1.9 A and 3.0 A, respectively. Features that can be clearly visualized at this high resolution include ATP binding with octahedral coordination of Mg(2+); an inner chamber that significantly changes in volume with the aid of tight connections among transmembrane helices (TM) 1, 3, and 6; a glutamate-arginine interaction that stabilizes the outward-facing conformation; and extensive interactions between TM1 and TM3, a property that distinguishes multidrug transporters from floppases. These structural elements are proposed to participate in the mechanism of the transporter. | ||
| + | |||
| + | Inward- and outward-facing X-ray crystal structures of homodimeric P-glycoprotein CmABCB1.,Kodan A, Yamaguchi T, Nakatsu T, Matsuoka K, Kimura Y, Ueda K, Kato H Nat Commun. 2019 Jan 8;10(1):88. doi: 10.1038/s41467-018-08007-x. PMID:30622258<ref>PMID:30622258</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6a6n" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Cyam1]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Kato, H]] | [[Category: Kato, H]] | ||
[[Category: Kodan, A]] | [[Category: Kodan, A]] | ||
Revision as of 21:26, 28 October 2020
Crystal structure of an inward-open apo state of the eukaryotic ABC multidrug transporter CmABCB1
| |||||||||||
