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| | ==Crystal structure of AL2 PAL domain in complex with AtRing1a proximal site== | | ==Crystal structure of AL2 PAL domain in complex with AtRing1a proximal site== |
| - | <StructureSection load='5y21' size='340' side='right' caption='[[5y21]], [[Resolution|resolution]] 1.77Å' scene=''> | + | <StructureSection load='5y21' size='340' side='right'caption='[[5y21]], [[Resolution|resolution]] 1.77Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5y21]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y21 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5y21]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y21 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AL2, At3g11200, F11B9.12, F9F8.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.769Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y21 OCA], [http://pdbe.org/5y21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y21 RCSB], [http://www.ebi.ac.uk/pdbsum/5y21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y21 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y21 OCA], [https://pdbe.org/5y21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y21 RCSB], [https://www.ebi.ac.uk/pdbsum/5y21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y21 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ALFL2_ARATH ALFL2_ARATH]] Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. | + | [https://www.uniprot.org/uniprot/ALFL2_ARATH ALFL2_ARATH] Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Huang, Y]] | + | [[Category: Large Structures]] |
| - | [[Category: Peng, L]] | + | [[Category: Huang Y]] |
| - | [[Category: Wang, L L]] | + | [[Category: Peng L]] |
| - | [[Category: Alfin-like family protein]] | + | [[Category: Wang LL]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Gene regulation]]
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| - | [[Category: Prc1 interactor]]
| + | |
| Structural highlights
Function
ALFL2_ARATH Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes.
Publication Abstract from PubMed
Polycomb group proteins play essential roles in transcriptional gene repression during both animal and plant development. Polycomb repression complex 1 (PRC1) is one of the key functional modules in polycomb group silencing. It acts as both a reader of H3K27me3 (histone H3 lysine 27 trimethylation) and a writer of H2Aub1 (histone H2A monoubiquitination) in establishing stable repression chromatin state. Intriguingly, a recent study showed that Arabidopsis PRC1 contains the H3K4me3-binding proteins of the ALFIN-like (AL) family, pointing to a chromatin state switch from active to repressive transcription of embryonic genes required for vegetative plant development. However, molecular and structural basis of AL-PRC1 complexes are lacking, which harmed insightful mechanistic understanding of AL-PRC1 complex function. In the present study, we report the crystal structures of the PAL domain (DUF3594 domain) of AL2 and AL7 proteins as well as their mechanistic binding to the PRC1 ring-finger proteins (RING1 and BMI1). We found that the PAL domain exists as a homodimer and represents a novel protein fold. We further determined the crystal structures of the PAL domain of AL2 (AL2-PAL) in complex with AtRING1a and AtBMI1b, the two core components of Arabidopsis PRC1. Interestingly, two PAL-binding sites were found on AtRING1a. Each of them can bind AL but with different affinities and distinct structural bases. Based on our results, we propose a mechanistic model to understand how AL proteins target PRC1 to active chromatin to undergo the transition from H3K4me3 to H2Aub1/H3K27me3 in establishing gene silencing.
Structural Analysis of the Arabidopsis AL2-PAL and PRC1 Complex Provides Mechanistic Insight into Active-to-Repressive Chromatin State Switch.,Peng L, Wang L, Zhang Y, Dong A, Shen WH, Huang Y J Mol Biol. 2018 Oct 19;430(21):4245-4259. doi: 10.1016/j.jmb.2018.08.021. Epub, 2018 Aug 31. PMID:30176245[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Peng L, Wang L, Zhang Y, Dong A, Shen WH, Huang Y. Structural Analysis of the Arabidopsis AL2-PAL and PRC1 Complex Provides Mechanistic Insight into Active-to-Repressive Chromatin State Switch. J Mol Biol. 2018 Oct 19;430(21):4245-4259. doi: 10.1016/j.jmb.2018.08.021. Epub, 2018 Aug 31. PMID:30176245 doi:http://dx.doi.org/10.1016/j.jmb.2018.08.021
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