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| | ==Crystal structure of LptC== | | ==Crystal structure of LptC== |
| - | <StructureSection load='3my2' size='340' side='right' caption='[[3my2]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='3my2' size='340' side='right'caption='[[3my2]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3my2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_mg1655 Escherichia coli mg1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MY2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MY2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3my2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MG1655 Escherichia coli str. K-12 substr. MG1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MY2 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b3199, JW3166, lptC, yrbK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511145 Escherichia coli MG1655])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3my2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3my2 OCA], [http://pdbe.org/3my2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3my2 RCSB], [http://www.ebi.ac.uk/pdbsum/3my2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3my2 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3my2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3my2 OCA], [https://pdbe.org/3my2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3my2 RCSB], [https://www.ebi.ac.uk/pdbsum/3my2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3my2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LPTC_ECOLI LPTC_ECOLI]] Required for the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. It facilitates the transfer of LPS from the inner membrane to a periplasmic chaperone, LptA.<ref>PMID:16765569</ref> <ref>PMID:18424520</ref> <ref>PMID:20720015</ref> | + | [https://www.uniprot.org/uniprot/LPTC_ECOLI LPTC_ECOLI] Required for the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. It facilitates the transfer of LPS from the inner membrane to a periplasmic chaperone, LptA.<ref>PMID:16765569</ref> <ref>PMID:18424520</ref> <ref>PMID:20720015</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/my/3my2_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/my/3my2_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Escherichia coli mg1655]] | + | [[Category: Escherichia coli str. K-12 substr. MG1655]] |
| - | [[Category: Dong, C]] | + | [[Category: Large Structures]] |
| - | [[Category: SSPF, Scottish Structural Proteomics Facility]] | + | [[Category: Dong C]] |
| - | [[Category: Tran, A X]] | + | [[Category: Tran AX]] |
| - | [[Category: Whitfield, C]] | + | [[Category: Whitfield C]] |
| - | [[Category: Lipopolysaccharide export pathway]]
| + | |
| - | [[Category: Lptc]]
| + | |
| - | [[Category: Scottish structural proteomics facility]]
| + | |
| - | [[Category: Sspf]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Transport protein]]
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| Structural highlights
Function
LPTC_ECOLI Required for the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. It facilitates the transfer of LPS from the inner membrane to a periplasmic chaperone, LptA.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
LptC is a conserved bitopic inner membrane protein from Escherichia coli involved in the export of lipopolysaccharide from its site of synthesis in the cytoplasmic membrane to the outer membrane. LptC forms a complex with the ATP-binding cassette transporter, LptBFG, which is thought to facilitate the extraction of lipopolysaccharide from the inner membrane and release it into a translocation pathway that includes the putative periplasmic chaperone LptA. Cysteine-modification experiments established that the catalytic domain of LptC is oriented towards the periplasm. The structure of the periplasmic domain is described at a resolution of 2.2-angstroms from X-ray crystallographic data. The periplasmic domain of LptC consists of a twisted-boat structure with two beta-sheets in apposition to each other. The beta-sheets contain seven and eight anti-parallel beta-strands, respectively. This structure bears a high degree of resemblance to the crystal structure of LptA. Like LptA, LptC binds lipopolysaccharide in vitro. In vitro, LptA can displace lipopolysaccharide from LptC (but not vice versa), consistent with their locations and their proposed placement in a unidirectional export pathway.
Structure and functional analysis of LptC, a conserved membrane protein involved in the lipopolysaccharide export pathway in Escherichia coll.,Tran AX, Dong C, Whitfield C J Biol Chem. 2010 Aug 18. PMID:20720015[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sperandeo P, Pozzi C, Deho G, Polissi A. Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus. Res Microbiol. 2006 Jul-Aug;157(6):547-58. Epub 2006 Feb 9. PMID:16765569 doi:10.1016/j.resmic.2005.11.014
- ↑ Sperandeo P, Lau FK, Carpentieri A, De Castro C, Molinaro A, Deho G, Silhavy TJ, Polissi A. Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli. J Bacteriol. 2008 Jul;190(13):4460-9. Epub 2008 Apr 18. PMID:18424520 doi:JB.00270-08
- ↑ Tran AX, Dong C, Whitfield C. Structure and functional analysis of LptC, a conserved membrane protein involved in the lipopolysaccharide export pathway in Escherichia coll. J Biol Chem. 2010 Aug 18. PMID:20720015 doi:10.1074/jbc.M110.144709
- ↑ Tran AX, Dong C, Whitfield C. Structure and functional analysis of LptC, a conserved membrane protein involved in the lipopolysaccharide export pathway in Escherichia coll. J Biol Chem. 2010 Aug 18. PMID:20720015 doi:10.1074/jbc.M110.144709
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