6nmc
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==CryoEM structure of the LbCas12a-crRNA-2xAcrVA1 complex== | |
| + | <StructureSection load='6nmc' size='340' side='right'caption='[[6nmc]], [[Resolution|resolution]] 4.24Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6nmc]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NMC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NMC FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nmc OCA], [http://pdbe.org/6nmc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nmc RCSB], [http://www.ebi.ac.uk/pdbsum/6nmc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nmc ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CRISPR-Cas12a (Cpf1), a type V CRISPR-associated nuclease, provides bacterial immunity against bacteriophages and plasmids but also serves as a tool for genome editing. Foreign nucleic acids are integrated into the CRISPR locus, prompting transcription of CRISPR RNAs (crRNAs) that guide Cas12a cleavage of foreign complementary DNA. However, mobile genetic elements counteract Cas12a with inhibitors, notably type V-A anti-CRISPRs (AcrVAs). We present cryoelectron microscopy structures of Cas12a-crRNA bound to AcrVA1 and AcrVA4 at 3.5 and 3.3 A resolutions, respectively. AcrVA1 is sandwiched between the recognition (REC) and nuclease (NUC) lobes of Cas12a and inserts into the binding pocket for the protospacer-adjacent motif (PAM), a short DNA sequence guiding Cas12a targeting. AcrVA1 cleaves crRNA in a Cas12a-dependent manner, inactivating Cas12a-crRNA complexes. The AcrVA4 dimer is anchored around the crRNA pseudoknot of Cas12a-crRNA, preventing required conformational changes for crRNA-DNA heteroduplex formation. These results uncover molecular mechanisms for CRISPR-Cas12a inhibition, providing insights into bacteria-phage dynamics. | ||
| - | + | Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins.,Zhang H, Li Z, Daczkowski CM, Gabel C, Mesecar AD, Chang L Cell Host Microbe. 2019 May 27. pii: S1931-3128(19)30249-5. doi:, 10.1016/j.chom.2019.05.004. PMID:31155345<ref>PMID:31155345</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6nmc" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chang, L]] | ||
| + | [[Category: Li, Z]] | ||
| + | [[Category: Zhang, H]] | ||
| + | [[Category: Unknown function-rna complex]] | ||
Revision as of 05:40, 12 June 2019
CryoEM structure of the LbCas12a-crRNA-2xAcrVA1 complex
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