6np6
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the sensor domain of the transcriptional regulator HcpR from Porphyromonas Gingivalis== | |
| + | <StructureSection load='6np6' size='340' side='right'caption='[[6np6]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6np6]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5v3e 5v3e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NP6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NP6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6np6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6np6 OCA], [http://pdbe.org/6np6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6np6 RCSB], [http://www.ebi.ac.uk/pdbsum/6np6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6np6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Although the HcpR regulator plays a vital step in initiation of the nitrosative stress response in many Gram-negative anaerobic bacteria, the molecular mechanisms that it uses to mediate gas sensing are not well understood. Here, a 2.6 A resolution crystal structure of the N-terminal sensing domain of the anaerobic periodontopathogen Porphyromonas gingivalis HcpR is presented. The protein has classical features of the regulators belonging to the FNR-CRP family and contains a hydrophobic pocket in its N-terminal sensing domain. It is shown that heme bound to HcpR exhibits heme iron as a hexacoordinate system in the absence of nitric oxide (NO) and that upon nitrosylation it transitions to a pentacoordinate system. Finally, small-angle X-ray scattering experiments on full-length HcpR reveal that the C-terminal DNA-binding domain of HcpR has a high degree of interdomain flexibility. | ||
| - | + | Nitrosative stress sensing in Porphyromonas gingivalis: structure of and heme binding by the transcriptional regulator HcpR.,Belvin BR, Musayev FN, Burgner J, Scarsdale JN, Escalante CR, Lewis JP Acta Crystallogr D Struct Biol. 2019 Apr 1;75(Pt 4):437-450. doi:, 10.1107/S205979831900264X. Epub 2019 Apr 5. PMID:30988260<ref>PMID:30988260</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Lewis, J | + | <div class="pdbe-citations 6np6" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Belvin, B R]] | ||
| + | [[Category: Escalante, C R]] | ||
| + | [[Category: Lewis, J P]] | ||
| + | [[Category: Musayev, F N]] | ||
| + | [[Category: Scarsdale, J N]] | ||
[[Category: Turner, J]] | [[Category: Turner, J]] | ||
| - | [[Category: | + | [[Category: Beta barrel]] |
| - | [[Category: | + | [[Category: Dimerization helix]] |
| - | [[Category: | + | [[Category: Heme binding protein]] |
| - | [[Category: | + | [[Category: Transcription]] |
| + | [[Category: Transcriptional regulator]] | ||
Revision as of 06:41, 26 June 2019
Crystal structure of the sensor domain of the transcriptional regulator HcpR from Porphyromonas Gingivalis
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