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9dna
From Proteopedia
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'''CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)''' | '''CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9DNA OCA]. | |
==Reference== | ==Reference== | ||
Crystal structure analysis of an A-DNA fragment at 1.8 A resolution: d(GCCCGGGC)., Heinemann U, Lauble H, Frank R, Blocker H, Nucleic Acids Res. 1987 Nov 25;15(22):9531-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3684603 3684603] | Crystal structure analysis of an A-DNA fragment at 1.8 A resolution: d(GCCCGGGC)., Heinemann U, Lauble H, Frank R, Blocker H, Nucleic Acids Res. 1987 Nov 25;15(22):9531-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3684603 3684603] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Heinemann, U.]] | [[Category: Heinemann, U.]] | ||
| - | [[Category: | + | [[Category: A-dna]] |
| - | [[Category: | + | [[Category: Double helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:52:47 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:52, 4 May 2008
CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)
Overview
Single crystals of the self-complementary octadeoxyribonucleotide d(GCCCGGGC) have been analysed by X-ray diffraction methods at a resolution of 1.8 A. The tetragonal unit cell of space group P4(3)2(1)2 has dimensions of a = 43.25 A and c = 24.61 A and contains eight strands of the oligonucleotide. The structure was refined by standard crystallographic techniques to an R factor of 17.1% using 1359 3 sigma structure factor observations. Two strands of the oligonucleotide are related by the crystallographic dyad axis to form a DNA helix in the A conformation. The d(GCCCGGGC) helix is characterized by a wide open major groove, a near perpendicular orientation of base pairs to the helix axis and an unusually small average helix twist angle of 31.3 degrees indicating a slightly underwound helix with 11.5 base pairs per turn. Extensive cross-strand stacking between guanine bases at the central cytosine-guanine step is made possible by a number of local conformational adjustments including a fully extended sugar-phosphate backbone of the central guanosine nucleotide.
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure analysis of an A-DNA fragment at 1.8 A resolution: d(GCCCGGGC)., Heinemann U, Lauble H, Frank R, Blocker H, Nucleic Acids Res. 1987 Nov 25;15(22):9531-50. PMID:3684603 Page seeded by OCA on Sun May 4 22:52:47 2008
