6c68
From Proteopedia
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==MHC-independent t cell receptor A11== | ==MHC-independent t cell receptor A11== | ||
| - | <StructureSection load='6c68' size='340' side='right' caption='[[6c68]], [[Resolution|resolution]] 2.59Å' scene=''> | + | <StructureSection load='6c68' size='340' side='right'caption='[[6c68]], [[Resolution|resolution]] 2.59Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6c68]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C68 OCA]. For a <b>guided tour on the structure components</b> use [http:// | + | <table><tr><td colspan='2'>[[6c68]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C68 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6C68 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6c68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c68 OCA], [http://pdbe.org/6c68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c68 RCSB], [http://www.ebi.ac.uk/pdbsum/6c68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c68 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | During normal T cell development in the thymus, alphabeta TCRs signal immature thymocytes to differentiate into mature T cells by binding to peptide-MHC ligands together with CD4/CD8 coreceptors. Conversely, in MHC and CD4/CD8 coreceptor-deficient mice, the thymus generates mature T cells expressing MHC-independent TCRs that recognize native conformational epitopes rather than linear antigenic-peptides presented by MHC. To date, no structural information of MHC-independent TCRs is available, and their structural recognition of non-MHC ligand remains unknown. To our knowledge in this study, we determined the first structures of two murine MHC-independent TCRs (A11 and B12A) that bind with high nanomolar affinities to mouse adhesion receptor CD155. Solution binding demonstrated the Valphabeta-domain is responsible for MHC-independent B12A recognition of its ligand. Analysis of A11 and B12A sequences against various MHC-restricted and -independent TCR sequence repertoires showed that individual V-genes of A11 and B12A did not exhibit preference against MHC-restriction. Likewise, CDR3 alone did not discriminate against MHC binding, suggesting VDJ recombination together with Valpha/Vbeta pairing determine their MHC-independent specificity for CD155. The structures of A11 and B12A TCR are nearly identical to those of MHC-restricted TCR, including the conformations of CDR1 and 2. Mutational analysis, together with negative-staining electron microscopy images, showed that the CDR regions of A11 and B12A recognized epitopes on D1 domain of CD155, a region also involved in CD155 binding to poliovirus and Tactile in human. Taken together, MHC-independent TCRs adopt canonical TCR structures to recognize native Ags, highlighting the importance of thymic selection in determining TCR ligand specificity. | ||
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| + | Structure of MHC-Independent TCRs and Their Recognition of Native Antigen CD155.,Lu J, Van Laethem F, Saba I, Chu J, Tikhonova AN, Bhattacharya A, Singer A, Sun PD J Immunol. 2020 Jun 15;204(12):3351-3359. doi: 10.4049/jimmunol.1901084. Epub, 2020 Apr 22. PMID:32321756<ref>PMID:32321756</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6c68" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lk3 transgenic mice]] | ||
[[Category: Arnon, T]] | [[Category: Arnon, T]] | ||
[[Category: Bhattacharya, A]] | [[Category: Bhattacharya, A]] | ||
Revision as of 09:53, 12 August 2020
MHC-independent t cell receptor A11
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Categories: Large Structures | Lk3 transgenic mice | Arnon, T | Bhattacharya, A | Chu, J | Friedman, N | Ko, A | Laethem, F Van | Love, N C | Lu, J | Radaev, S | Saba, I | Shifrut, E | Singer, A | Sun, P D | Sun, X | Tikhonova, A | Weng, N | Immune system | Mhc-independent t cell receptor
