This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

6ift

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 09:53, 13 February 2019

KsgA from Bacillus subtilis in complex with SAM

Structural highlights

6ift is a 1 chain structure with sequence from Bacsu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	rsmA, ksgA, BSU00420 (BACSU)
Activity:	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase, with EC number 2.1.1.182
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RSMA_BACSU] Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.[HAMAP-Rule:MF_00607]

Publication Abstract from PubMed

Post-translational methylation of ribosomal RNA at select positions is a prevalent resistance mechanism adopted by pathogens. In this work, KsgA, a housekeeping ribosomal methyltransferase (rMtase) involved in ribosome biogenesis, was exploited as a model system to delineate the specific targeting determinants that impart substrate specificity to rMtases. With a combination of evolutionary and structure-guided approaches, a set of chimeras were created that altered the targeting specificity of KsgA such that it acted similarly to erythromycin-resistant methyltransferases (Erms), rMtases found in multidrug-resistant pathogens. The results revealed that specific loop embellishments on the basic Rossmann fold are key determinants in the selection of the cognate RNA. Moreover, in vivo studies confirmed that chimeric constructs are competent in imparting macrolide resistance. This work explores the factors that govern the emergence of resistance and paves the way for the design of specific inhibitors useful in reversing antibiotic resistance.

Deciphering Determinants in Ribosomal Methyltransferases that Confer Antimicrobial Resistance.,Bhujbalrao R, Anand R J Am Chem Soc. 2019 Jan 9. doi: 10.1021/jacs.8b10277. PMID:30624914^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bhujbalrao R, Anand R. Deciphering Determinants in Ribosomal Methyltransferases that Confer Antimicrobial Resistance. J Am Chem Soc. 2019 Jan 9. doi: 10.1021/jacs.8b10277. PMID:30624914 doi:http://dx.doi.org/10.1021/jacs.8b10277

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ift"

@@ Line 3: / Line 3: @@
 <StructureSection load='6ift' size='340' side='right' caption='[[6ift]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ift]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IFT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ift]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IFT FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rsmA, ksgA, BSU00420 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/16S_rRNA_(adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.182 2.1.1.182] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ift FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ift OCA], [http://pdbe.org/6ift PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ift RCSB], [http://www.ebi.ac.uk/pdbsum/6ift PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ift ProSAT]</span></td></tr>
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Bacsu]]
 [[Category: Anand, R]]
 [[Category: Bhujbalrao, R]]

6ift

From Proteopedia

Revision as of 09:53, 13 February 2019

KsgA from Bacillus subtilis in complex with SAM

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox