6iuj

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Revision as of 07:07, 27 March 2019

Crystal structure of GH30 xylanase B from Talaromyces cellulolyticus

Structural highlights

6iuj is a 2 chain structure with sequence from Acremonium cellulolyticus cf-2612. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Glucuronoxylanases are endo-xylanases and members of the glycoside hydrolase family 30 subfamilies7 (GH30-7) and 8 (GH30-8). Unlike for the well-studied GH30-8 enzymes, the structural and functional characteristics of GH30-7 enzymes remain poorly understood. Here, we report the catalytic properties and 3D structure of GH30-7 xylanase B (Xyn30B) identified from the cellulolytic fungus Talaromyces cellulolyticus Xyn30B efficiently degraded glucuronoxylan to acidic xylooligosaccharides (XOS), including an alpha-1,2-linked 4-O-methyl-D-glucuronosyl substituent (MeGlcA). Rapid analysis with negative-mode electrospray-ionization multistage MS (ESI(-)-MS(n)) revealed that the structures of the acidic XOS products are the same as those of the hydrolysates (MeGlcA(2)Xyln, n > 2) obtained with typical glucuronoxylanases. Acidic XOS products were further degraded by Xyn30B, releasing first xylobiose and then xylotetraose and xylohexaose as transglycosylation products. This hydrolase reaction was unique to Xyn30B, and the substrate was cleaved at the xylobiose unit from its non-reducing end, indicating that Xyn30B is a bifunctional enzyme possessing both endo-glucuronoxylanase and exo-xylobiohydrolase activities. The crystal structure of Xyn30B was determined as the first structure of a GH30-7 xylanase at 2.25 A resolution, revealing that Xyn30B is composed of a pseudo-(alpha/beta)8-catalytic domain, lacking an alpha6 helix, and a small beta-rich domain. This structure and site-directed mutagenesis clarified that Arg-46, conserved in GH30-7 glucuronoxylanases, is a critical residue for MeGlcA appendage-dependent xylan degradation. The structural comparison between Xyn30B and the GH30-8 enzymes suggests that Asn-93 in the beta2-alpha2 loop is involved in xylobiohydrolase activity. In summary, our findings indicate that Xyn30B is a bifunctional endo- and exo-xylanase.

Structural and functional characterization of a bifunctional GH30-7 xylanase B from the filamentous fungus Talaromyces cellulolyticus.,Nakamichi Y, Fouquet T, Ito S, Watanabe M, Matsushika A, Inoue H J Biol Chem. 2019 Jan 17. pii: RA118.007207. doi: 10.1074/jbc.RA118.007207. PMID:30655295^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nakamichi Y, Fouquet T, Ito S, Watanabe M, Matsushika A, Inoue H. Structural and functional characterization of a bifunctional GH30-7 xylanase B from the filamentous fungus Talaromyces cellulolyticus. J Biol Chem. 2019 Jan 17. pii: RA118.007207. doi: 10.1074/jbc.RA118.007207. PMID:30655295 doi:http://dx.doi.org/10.1074/jbc.RA118.007207

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6iuj"

@@ Line 1: / Line 1: @@
 ==Crystal structure of GH30 xylanase B from Talaromyces cellulolyticus==
-<StructureSection load='6iuj' size='340' side='right' caption='[[6iuj]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+<StructureSection load='6iuj' size='340' side='right'caption='[[6iuj]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6iuj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IUJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IUJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6iuj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Acremonium_cellulolyticus_cf-2612 Acremonium cellulolyticus cf-2612]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IUJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IUJ FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iuj OCA], [http://pdbe.org/6iuj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iuj RCSB], [http://www.ebi.ac.uk/pdbsum/6iuj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iuj ProSAT]</span></td></tr>
@@ Line 20: / Line 20: @@
 __TOC__
 </StructureSection>
+[[Category: Acremonium cellulolyticus cf-2612]]
+[[Category: Large Structures]]
 [[Category: Inoue, H]]
 [[Category: Nakamichi, Y]]

6iuj

From Proteopedia

Revision as of 07:07, 27 March 2019

Crystal structure of GH30 xylanase B from Talaromyces cellulolyticus

Structural highlights

Publication Abstract from PubMed

References

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