2oqd
From Proteopedia
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'''Crystal Structure of BthTX-II''' | '''Crystal Structure of BthTX-II''' | ||
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Crystal structure of a myotoxic Asp49-phospholipase A(2) with low catalytic activity: Insights into Ca(2+)-independent catalytic mechanism., Correa LC, Marchi-Salvador DP, Cintra AC, Sampaio SV, Soares AM, Fontes MR, Biochim Biophys Acta. 2008 Apr;1784(4):591-9. Epub 2008 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18261474 18261474] | Crystal structure of a myotoxic Asp49-phospholipase A(2) with low catalytic activity: Insights into Ca(2+)-independent catalytic mechanism., Correa LC, Marchi-Salvador DP, Cintra AC, Sampaio SV, Soares AM, Fontes MR, Biochim Biophys Acta. 2008 Apr;1784(4):591-9. Epub 2008 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18261474 18261474] | ||
[[Category: Bothrops jararacussu]] | [[Category: Bothrops jararacussu]] | ||
| - | [[Category: Phospholipase A(2)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cintra, A C.O.]] | [[Category: Cintra, A C.O.]] | ||
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[[Category: Marchi-Salvador, D P.]] | [[Category: Marchi-Salvador, D P.]] | ||
[[Category: Soares, A M.]] | [[Category: Soares, A M.]] | ||
| - | [[Category: | + | [[Category: Asp49-phospholipase a2]] |
| - | [[Category: | + | [[Category: Bothrops jararacussu]] |
| - | [[Category: | + | [[Category: Bothropstoxin ii]] |
| - | [[Category: | + | [[Category: Bthtx-ii]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:27:09 2008'' | |
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Revision as of 08:27, 4 May 2008
Crystal Structure of BthTX-II
Overview
A myotoxic Asp49-phospholipase A(2) (Asp49-PLA(2)) with low catalytic activity (BthTX-II from Bothrops jararacussu venom) was crystallized and the molecular-replacement solution has been obtained with a dimer in the asymmetric unit. The quaternary structure of BthTX-II resembles the myotoxic Asp49-PLA(2) PrTX-III (piratoxin III from B. pirajai venom) and all non-catalytic and myotoxic dimeric Lys49-PLA(2)s. Despite of this, BthTX-II is different from the highly catalytic and non-myotoxic BthA-I (acidic PLA(2) from B. jararacussu) and other Asp49-PLA(2)s. BthTX-II structure showed a severe distortion of calcium-binding loop leading to displacement of the C-terminal region. Tyr28 side chain, present in this region, is in an opposite position in relation to the same residue in the catalytic activity Asp49-PLA(2)s, making a hydrogen bond with the atom Odelta2 of the catalytically active Asp49, which should coordinate the calcium. This high distortion may also be confirmed by the inability of BthTX-II to bind Na(+) ions at the Ca(2+)-binding loop, despite of the crystallization to have occurred in the presence of this ion. In contrast, other Asp49-PLA(2)s which are able to bind Ca(2+) ions are also able to bind Na(+) ions at this loop. The comparison with other catalytic, non-catalytic and inhibited PLA(2)s indicates that the BthTX-II is not able to bind calcium ions; consequently, we suggest that its low catalytic function is based on an alternative way compared with other PLA(2)s.
About this Structure
2OQD is a Single protein structure of sequence from Bothrops jararacussu. Full crystallographic information is available from OCA.
Reference
Crystal structure of a myotoxic Asp49-phospholipase A(2) with low catalytic activity: Insights into Ca(2+)-independent catalytic mechanism., Correa LC, Marchi-Salvador DP, Cintra AC, Sampaio SV, Soares AM, Fontes MR, Biochim Biophys Acta. 2008 Apr;1784(4):591-9. Epub 2008 Jan 26. PMID:18261474 Page seeded by OCA on Sun May 4 11:27:09 2008
