6ahw

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==Crystal structure of circular-permutated YibK methyltransferase from Haemophilus influenzae==
==Crystal structure of circular-permutated YibK methyltransferase from Haemophilus influenzae==
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<StructureSection load='6ahw' size='340' side='right' caption='[[6ahw]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
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<StructureSection load='6ahw' size='340' side='right'caption='[[6ahw]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6ahw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Haein Haein]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AHW FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6ahw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae_Rd_KW20 Haemophilus influenzae Rd KW20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AHW FirstGlance]. <br>
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</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA_(cytidine(34)-2'-O)-methyltransferase tRNA (cytidine(34)-2'-O)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.207 2.1.1.207] </span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ahw OCA], [http://pdbe.org/6ahw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ahw RCSB], [http://www.ebi.ac.uk/pdbsum/6ahw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ahw ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ahw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ahw OCA], [https://pdbe.org/6ahw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ahw RCSB], [https://www.ebi.ac.uk/pdbsum/6ahw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ahw ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TRML_HAEIN TRML_HAEIN]] Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.[HAMAP-Rule:MF_01885]
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[https://www.uniprot.org/uniprot/TRML_HAEIN TRML_HAEIN] Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.[HAMAP-Rule:MF_01885]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Topologically knotted proteins are tantalizing examples of how polypeptide chains can explore complex free energy landscapes to efficiently attain defined knotted conformations. The evolution trails of protein knots, however, remain elusive. We used circular permutation to change an evolutionally conserved topologically knotted SPOUT RNA methyltransferase into an unknotted form. The unknotted variant adopted the same three-dimensional structure and oligomeric state as its knotted parent, but its folding stability was markedly reduced with accelerated folding kinetics and its ligand binding was abrogated. Our findings support the hypothesis that the universally conserved knotted topology of the SPOUT superfamily evolved from unknotted forms through circular permutation under selection pressure for folding robustness and, more importantly, for functional requirements associated with the knotted structural element.
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Untying a Protein Knot by Circular Permutation.,Chuang YC, Hu IC, Lyu PC, Hsu SD J Mol Biol. 2019 Jan 9. pii: S0022-2836(19)30007-5. doi:, 10.1016/j.jmb.2019.01.005. PMID:30639189<ref>PMID:30639189</ref>
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==See Also==
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*[[TRNA methyltransferase 3D structures|TRNA methyltransferase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6ahw" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Haein]]
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[[Category: Haemophilus influenzae Rd KW20]]
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[[Category: Chuang, Y C]]
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[[Category: Large Structures]]
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[[Category: Hsu, S T.D]]
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[[Category: Chuang YC]]
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[[Category: Lyu, P C]]
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[[Category: Hsu STD]]
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[[Category: Circular permutation]]
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[[Category: Lyu PC]]
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[[Category: Methyltransferase]]
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[[Category: Transferase]]
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Current revision

Crystal structure of circular-permutated YibK methyltransferase from Haemophilus influenzae

PDB ID 6ahw

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