2cc3
From Proteopedia
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| - | [[Image:2cc3.gif|left|200px]]<br /> | + | [[Image:2cc3.gif|left|200px]]<br /><applet load="2cc3" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2cc3" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2cc3, resolution 2.20Å" /> | caption="2cc3, resolution 2.20Å" /> | ||
'''STRUCTURE OF AGROBACTERIUM TUMEFACIENS VIRB8 PROTEIN'''<br /> | '''STRUCTURE OF AGROBACTERIUM TUMEFACIENS VIRB8 PROTEIN'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2CC3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens] with MPD as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 2CC3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens] with MPD as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Mpd Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CC3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: virb8]] | [[Category: virb8]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:17:25 2007'' |
Revision as of 17:07, 18 December 2007
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STRUCTURE OF AGROBACTERIUM TUMEFACIENS VIRB8 PROTEIN
Overview
Bacterial type IV secretion systems (T4SS) translocate DNA and/or proteins, to recipient cells, thus providing a mechanism for conjugative transfer of, genetic material and bacterial pathogenesis. Here we describe the first, structure of a core component from the archetypal Agrobacterium, tumefaciens T4SS: the 2.2-A resolution crystal structure of the VirB8, periplasmic domain (pVirB8(AT)). VirB8 forms a dimer in the crystal, and, we identify residues likely important for stabilization of the dimer, interface. Structural comparison of pVirB8(AT) with Brucella suis VirB8, confirms that the monomers have a similar fold. In addition, the, pVirB8(AT) dimer superimposes very closely on the B. suis VirB8 dimer, supporting the proposal that dimer formation in the crystal reflects, self-interactions that are biologically significant. The evolutionary, conservation level for each residue was obtained from a data set of 84, VirB8 homologs and projected onto the protein structure to indicate, conserved surface patches that likely contact other T4SS proteins.
About this Structure
2CC3 is a Single protein structure of sequence from Agrobacterium tumefaciens with MPD as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Agrobacterium tumefaciens VirB8 structure reveals potential protein-protein interaction sites., Bailey S, Ward D, Middleton R, Grossmann JG, Zambryski PC, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2582-7. Epub 2006 Feb 15. PMID:16481621
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