3bjx

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(New page: 200px {{Structure |PDB= 3bjx |SIZE=350|CAPTION= <scene name='initialview01'>3bjx</scene>, resolution 2.300&Aring; |SITE= <scene name='pdbsite=AC1:So4+Binding+Site...)
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[[Image:3bjx.jpg|left|200px]]
[[Image:3bjx.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 3bjx |SIZE=350|CAPTION= <scene name='initialview01'>3bjx</scene>, resolution 2.300&Aring;
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The line below this paragraph, containing "STRUCTURE_3bjx", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+297'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+B+297'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+C+297'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+D+297'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+B+298'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+D+298'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+A+298'>AC7</scene>, <scene name='pdbsite=AC8:So4+Binding+Site+For+Residue+D+299'>AC8</scene>, <scene name='pdbsite=AC9:So4+Binding+Site+For+Residue+C+298'>AC9</scene>, <scene name='pdbsite=BC1:So4+Binding+Site+For+Residue+A+299'>BC1</scene>, <scene name='pdbsite=BC2:So4+Binding+Site+For+Residue+D+300'>BC2</scene>, <scene name='pdbsite=BC3:So4+Binding+Site+For+Residue+C+299'>BC3</scene>, <scene name='pdbsite=BC4:So4+Binding+Site+For+Residue+C+300'>BC4</scene>, <scene name='pdbsite=BC5:So4+Binding+Site+For+Residue+B+299'>BC5</scene>, <scene name='pdbsite=BC6:So4+Binding+Site+For+Residue+D+301'>BC6</scene> and <scene name='pdbsite=BC7:So4+Binding+Site+For+Residue+A+300'>BC7</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/(S)-2-haloacid_dehalogenase (S)-2-haloacid dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.2 3.8.1.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= dehI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])
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-->
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|DOMAIN=
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{{STRUCTURE_3bjx| PDB=3bjx | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjx OCA], [http://www.ebi.ac.uk/pdbsum/3bjx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bjx RCSB]</span>
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}}
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'''Structure of a Group I haloacid dehalogenase from Pseudomonas putida strain PP3'''
'''Structure of a Group I haloacid dehalogenase from Pseudomonas putida strain PP3'''
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==Reference==
==Reference==
The Crystal Structure of DehI Reveals a New alpha-Haloacid Dehalogenase Fold and Active-Site Mechanism., Schmidberger JW, Wilce JA, Weightman AJ, Whisstock JC, Wilce MC, J Mol Biol. 2008 Feb 29;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18353360 18353360]
The Crystal Structure of DehI Reveals a New alpha-Haloacid Dehalogenase Fold and Active-Site Mechanism., Schmidberger JW, Wilce JA, Weightman AJ, Whisstock JC, Wilce MC, J Mol Biol. 2008 Feb 29;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18353360 18353360]
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[[Category: (S)-2-haloacid dehalogenase]]
 
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Schmidberger, J W.]]
[[Category: Schmidberger, J W.]]
[[Category: Wilce, M C.J.]]
[[Category: Wilce, M C.J.]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: plasmid]]
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[[Category: Plasmid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:33:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 2 11:59:16 2008''
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Revision as of 06:33, 24 April 2008

Image:3bjx.jpg

Template:STRUCTURE 3bjx

Structure of a Group I haloacid dehalogenase from Pseudomonas putida strain PP3


Overview

Haloacid dehalogenases catalyse the removal of halides from organic haloacids and are of interest for bioremediation and for their potential use in the synthesis of industrial chemicals. We present the crystal structure of the homodimer DehI from Pseudomonas putida strain PP3, the first structure of a group I alpha-haloacid dehalogenase that can process both l- and d-substrates. The structure shows that the DehI monomer consists of two domains of approximately 130 amino acids that have approximately 16% sequence identity yet adopt virtually identical and unique folds that form a pseudo-dimer. Analysis of the active site reveals the likely binding mode of both l- and d-substrates with respect to key catalytic residues. Asp189 is predicted to activate a water molecule for nucleophilic attack of the substrate chiral centre resulting in an inversion of configuration of either l- or d-substrates in contrast to d-only enzymes. These details will assist with future bioengineering of dehalogenases.

About this Structure

3BJX is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

The Crystal Structure of DehI Reveals a New alpha-Haloacid Dehalogenase Fold and Active-Site Mechanism., Schmidberger JW, Wilce JA, Weightman AJ, Whisstock JC, Wilce MC, J Mol Biol. 2008 Feb 29;. PMID:18353360 Page seeded by OCA on Thu Apr 24 09:33:06 2008

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